Synthesis of S-[13N]nitrosoglutathione (13N-GSNO) as a new potential PET imaging agent |
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| Affiliation: | 1. Ottawa Hospital Research Institute, General Campus, 501 Smyth Road, Ottawa, ON K1H 8L6, Canada;2. Department of Cellular and Molecular Medicine, University of Ottawa, 451 Smyth Road, Ottawa, ON K1H 8M5, Canada;3. Department of Anaesthesiology, Yale University, 10 Amistad Rd, New Haven, CT 06519, United States;1. CITHEFOR EA3452 “Drug targets, formulation and preclinical assessment”, Faculté de Pharmacie, Université de Lorraine, Nancy, France;2. State Key Laboratory of Virology, Ministry of Education Key Laboratory of Combinatorial Biosynthesis and Drug Discovery, Wuhan University School of Pharmaceutical Sciences, Wuhan, China |
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| Abstract: | In the present paper, a fast and reproducible method for the synthesis of S-[13N]nitrosoglutathione is reported for the first time. The labeling strategy is based on the production of [13N]NO3− via the 16O(p,α)13N nuclear reaction in water, as opposed to the standardized production of [13N]NH4+ in 2 mM aqueous ethanol. Following the reduction of [13N]NO3− to [13N]NO2−, the reaction with glutathione in the presence of hydrochloric acid led to the desired radiotracer with a good radiochemical yield (24.2±2.0% end of synthesis, n=5) in a short production time (3 min from the end of bombardment). |
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