顶空进样-气相色谱-质谱法测定芪白平肺胶囊中挥发性成分

目的 测定芪白平肺胶囊中挥发性成分.方法 采用顶空进样气相色谱质谱联用技术,并通过所用质谱库检索研究其挥发性化学成分组成,用面积归一化法确定各成分的相对百分含量.结果 共分辨出68个色谱峰,鉴定出53个化学成分,约占总挥发性成分含量的90.75,主要挥发性成分为酯类（38.58％）、酸类（22.97％）、杂环类（13.54％）、醛类（8.75％）、醇类（2.84％）、醚类（2.71％）、芳烃类（0.74％）和酮类（0.62％）物质,其中含量最高的是乙酸乙酯（25.54％）,其次为乙酸（11.79％）,含量较高的为2-甲基丁醛（7.00％）,2-氨基丁酸（6.83％）,乙酸丙酯（5.60％）,2,3-二羟基丙酸（3.45％）,糠醛（3.08％）,5-甲基糠醛（3.05％）,2甲基四氢呋喃-3-酮（2.82％）,2乙酰基呋喃（2.54％）,甲基环氧丙烷（2.03％）,等.结论 所建立的方法分离效果较好,能够准确鉴定芪白平肺胶囊中主要挥发性成分.

Determination of Volatile Components in Qibai Pingfei Capsule by Headspace-Gas Chromatography-Mass Spectrometry

Objective To determine the volatile components in Qibai Pingfei Capsule. Methods The volatile components in Qibai Pingfei Capsule were identified by headspace-gas chromatography-mass spectrometry and NIST Mass Spectral Database, and the content of each component was determined by area normalization. Results Sixty-eight chromatographic peaks were obtained, and 53 compounds were identified, accounting for 90.75% of all volatile components. The main volatile components were ester (38.58%), acid (22.97%), heterocycle (13.54%), aldehyde (8.75%), alcohol (2.84%), ether (2.71%), arene (0.74%), and ketone (0.62%). Ethyl acetate had the highest content (25.54%), followed by acetic acid (11.79%), 2-methylbutyraldehyde (7.00%), 2-aminobutyric acid (6.83%), propyl acetate (5.60%), 2,3-dihydroxypropanoic acid (3.45%), furaldehyde (3.08%), 5-methylfurfural (3.05%), 2-methyltetrahydrofuran-3-one (2.82%), 2-acetylfuran (2.54%), and isobutylene epoxide (2.03%). Conclusion With the established method, the main volatile components in Qibai Pingfei Capsule can be well separated and accurately identified.