双调蛋白在人体子宫内膜组织中的表达特征

目的:研究双调蛋白在人体子宫内膜组织中的表达特征.方法:取增殖期和分泌期的人体子宫内膜,采用实时定量RT-PCR方法,检测双调蛋白mRNA在子宫内膜增殖期和分泌期的表达差异;采用原位杂交和免疫组织化学的方法,从基因和蛋白水平检测双调蛋白在人体子官内膜组织中的表达特征.结果:定量RT-PCR结果显示,双调蛋白mRNA在增殖期和分泌期的子宫内膜均有表达,在分泌期其表达量显著升高,是增殖期的32倍.原位杂交和免疫组织化学结果显示,双调蛋白主要分布于子宫内膜腺体,定位于细胞浆内;双调蛋白mRNA在增殖期和分泌期子宫内膜的表达分别为0.54±0.22和2.96±0.47(P<0.01);双调蛋白在增殖期和分泌期子宫内膜的表达分别为0.77±0.47和2.60±0.43(P<0.01).双调蛋白在分泌期子宫内膜中的表达显著升高,与荧光定量RT-PCR分析的结果相符.结论:双调蛋白在子宫内膜分泌期的表达显著增加,提示该蛋白可能与子宫内膜细胞分化或胚胎着床有关.

Expression of amphiregulin in human endometrium during the menstrual cycle

OBJECTIVE: To investigate the expression of amphiregulin in human endometrium during the menstrual cycle. METHODS: Endometrial tissues were collected from the patients undergoing hysterectomy or endometrial biopsy. Real-time RT-PCR, in situ hybridization and immunohistochemistry were used to detect the expression characteristics of amphiregulin in human endometrium in proliferative and secretory phases. RESULTS: Real-time RT-PCR showed the expression of amphiregulin mRNA in secretory phase was 32 times that in proliferative phase. The results from in situ hybridization and immunohistochemistry showed that amphiregulin was located in the cytoplasm and mainly expressed in the gland of endometrium. The expressions of amphiregulin mRNA in proliferative and secretory phases were 0.54+/-0.22 and 2.96+/-0.47 (P<0.01), and the expressions of amphiregulin protein in proliferative and secretory phases were 0.77+/-0.47 and 2.60+/-0.43 (P<0.01), respectively. The data demonstrated that the amphiregulin expression was significantly increased in secretory phase, which was consistent with the results from RT-PCR. CONCLUSION: The expression of amphiregulin was increased in the secretory phase of human endometrium, which suggests that amphiregulin may play an important role in human implantation.