Enhancing the utility of a prM/E-expressing chimeric vaccine for Japanese encephalitis by addition of the JEV NS1 gene |
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Authors: | Ishikawa Tomohiro Wang Gongbo Widman Douglas G Infante Ernesto Winkelmann Evandro R Bourne Nigel Mason Peter W |
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Affiliation: | a Department of Pathology, University of Texas Medical Branch (UTMB), 301 University Boulevard, Galveston, TX 77555-0436, USA b Department of Microbiology and Immunology, UTMB, Galveston, TX, USA c Department of Pediatrics, UTMB, Galveston, TX, USA d Sealy Center for Vaccine Development, UTMB, Galveston, TX, USA |
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Abstract: | Recently, we demonstrated that a single-cycle West Nile virus (WNV) named RepliVAX WN could be used to produce a chimeric Japanese encephalitis (JE) vaccine (RepliVAX JE) by replacing the WNV prM/E genes with those of JEV. Here, we tested if replacement of WNV NS1 gene in RepliVAX JE with that of JEV (producing TripliVAX JE) could produce a superior vaccine. TripliVAX JE elicited higher anti-E immunity and displayed better efficacy in mice than RepliVAX JE. Furthermore, TripliVAX JE displayed reduced immune interference caused by pre-existing anti-NS1 immunity. Thus, we propose prM/E/NS1 chimerization as a new strategy for flavivirus vaccine development. |
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Keywords: | Japanese encephalitis Vaccine Chimera |
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