Efficacy of a vaccine that links viral epitopes to flagellin in protecting aged mice from influenza viral infection |
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Authors: | Leng Jin Stout-Delgado Heather W Kavita Uma Jacobs Andrea Tang Jie Du Wei Tussey Lynda Goldstein Daniel R |
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Affiliation: | a Department of Internal Medicine and Immunobiology, Yale University of Medicine, New Haven, CT, USA b VaxInnate Corporation, Cranbury NJ, USA |
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Abstract: | Influenza vaccines are less effective in older people than younger people. This impaired ability to protect older people from influenza viral lung infection has important implications as older people suffer a higher morbidity and mortality from influenza viral lung infection than younger people. Therefore, the development of novel effective vaccines that induce protection from influenza viral infections in older people are urgently needed. We had previously shown that direct linking the TLR5 activator, flagellin, to viral peptides induces effective immunity to viral antigens in young mice and people, respectively. In this study, we tested the efficacy of this vaccine platform with the hemagglutinin peptide of the influenza A strain virus (vaccine denoted as STF2.HA1-2) in protecting aged mice from subsequent influenza viral lung infection. We found that a 3.0 μg dose of the vaccine was effective in reducing mortality and increasing clinical well-being during influenza viral lung infection in aged mice. However, this effect was inferior to the response induced in young mice. Defects in the adaptive immune system but not the innate immune system were associated with this reduced effectiveness of the vaccine with aging. Our results indicate that the STF2.HA1-2 vaccine is effective in protecting aged hosts from influenza lung infection, although defects in the adaptive immune system with aging may limit the effectiveness of this vaccine in older people. |
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Keywords: | BMDC, bone marrow derived dendritic cell DC, dendritic cell TLR, toll like receptor |
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