Evaluation of cell-mediated immune responses to two BCG vaccination regimes in young children in South Korea |
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Authors: | Hyejon Lee Sang Nae ChoHee Jin Kim Young Min AnhJi Eun Choi Chang Hwi KimPark Jae Ock Sung Hee OhDeok Ryun Kim Sian FloydHazel M Dockrell |
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Institution: | a Department of Technical Cooperation, Korean Institute of Tuberculosis, Seoul 121-150, Republic of Korea b Department of Microbiology and Institute of Immunology and Immunological Diseases, Yonsei University College of Medicine, Seoul 102-752, Republic of Korea c Department of Pediatrics, Eulji General Hospital, Seoul 139-872, Republic of Korea d Department of Pediatrics, Seoul National University Boramae Medical Center, Seoul 156-707, Republic of Korea e Department of Pediatrics, Soon Chun Hyang University Bucheon Hospital, Seoul 420-767, Republic of Korea f Department of Pediatrics, Han Yang University Medical Center Hospital, Seoul 133-792, Republic of Korea g Translational Research Division, International Vaccine Institute, Seoul 151-919, Republic of Korea h Department of Infectious Disease Epidemiology, London School of Hygiene and Tropical Medicine, London WC1E 7HT, UK i Department of Immunology and Infection, London School of Hygiene and Tropical Medicine, London WC1E 7HT, UK |
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Abstract: | Children in South Korea are vaccinated with either BCG Pasteur vaccine intradermally (ID), or with BCG Tokyo vaccine given by multipuncture device (MP). Data from a recent national survey indicated that in children under 6 years old, 31.1% had received the ID vaccine and 64.5% the MP vaccine. To compare the T cell responses induced by the two vaccines, children aged 3-7 were recruited and tested for tuberculin skin test reactivity and for in vitro IFN-γ responses to mycobacterial antigens. DTH responses were not significantly different in children vaccinated by either the ID or MP vaccines. PPD-induced IFN-γ was measured in supernatants of 6-day diluted whole blood cultures. IFN-γ production to PPD was not significantly different in the two vaccine groups, although there is a trend that the MP group gives a higher proportion of IFN-γ positivity than the ID group. In addition, when IFN-γ responses to the antigens ESAT-6 and CFP-10 were assessed in the 6-7 year old group, there was no significant difference between the two vaccine groups. Thus, there was no evidence that the increasing use of MP vaccination has reduced protection against M. tuberculosis in young children in South Korea, based on immunogenicity as assessed by DTH and IFN-γ responses to PPD, and also equivalent frequency of responses to ESAT-6 and CFP-10. |
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Keywords: | BCG vaccination Interferon-gamma release assay Tuberculin skin test Whole blood assay Multipuncture vaccination Intradermal vaccination Young children |
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