Prevention of influenza virus shedding and protection from lethal H1N1 challenge using a consensus 2009 H1N1 HA and NA adenovirus vector vaccine |
| |
| Authors: | Jones Frank R Gabitzsch Elizabeth S Xu Younong Balint Joseph P Borisevich Viktoriya Smith Jennifer Smith Jeanon Peng Bi-Hung Walker Aida Salazar Magda Paessler Slobodan |
| |
| Institution: | a Etubics Corporation, Seattle, WA 98199, USA
b Galveston National Laboratory, Department of Pathology, Sealy Vaccine Center, University of Texas Medical Branch, Galveston, TX, USA |
| |
| Abstract: | Vaccines against emerging pathogens such as the 2009 H1N1 pandemic virus can benefit from current technologies such as rapid genomic sequencing to construct the most biologically relevant vaccine. A novel platform (Ad5 E1-, E2b-]) has been utilized to induce immune responses to various antigenic targets. We employed this vector platform to express hemagglutinin (HA) and neuraminidase (NA) genes from 2009 H1N1 pandemic viruses. Inserts were consensuses sequences designed from viral isolate sequences and the vaccine was rapidly constructed and produced. Vaccination induced H1N1 immune responses in mice, which afforded protection from lethal virus challenge. In ferrets, vaccination protected from disease development and significantly reduced viral titers in nasal washes. H1N1 cell mediated immunity as well as antibody induction correlated with the prevention of disease symptoms and reduction of virus replication. The Ad5 E1-, E2b-] should be evaluated for the rapid development of effective vaccines against infectious diseases. |
| |
| Keywords: | Viral shedding Influenza H1N1 vaccine Adenovirus vector Ad5 [E1- E2b-] Hemagglutinin Neuraminidase Cell mediated immunity Pandemic Horizontal transmission |
| 本文献已被 ScienceDirect PubMed 等数据库收录！ |
|
