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Terminal stages of complement hemolysis: Technical comment |
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| Authors: | George H. Wirtz Shirley S. Westfall |
| Affiliation: | Department of Biochemistry, School of Medicine, West Virginia University, Morgantown, WV 26506, U.S.A. |
| Abstract: | In studying terminallytic events of sensitized erythrocytes which have reacted with all 9 components of complement, we agree in general with the conclusions of Boyle and Borsos [1] that the cellular intermediate passes through the stages E1bouned, E1inserted, and E1doomed. However, we differ with these workers on the influence of 3 variables on the terminal reactions. These variables are: time of hemolysis; temperature of E1 preparation; and the use of EDTA to manipulate E1. |
| Keywords: | E sheep erythrocytes EA sheep erythrocytes sensitized with anti-Forsmann antibody EAC1–7 EA which has reacted with complement components C1 through C7 EAC1–8 and EAC1–9 analogous definitions EA which has reacted with all 9 complement components but has not yet lysed (synonym of EAC1–9) Hb hemoglobin |
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