线粒体DNA缺失对人乳腺癌MDA-MB-231细胞药物敏感性的影响

目的 探讨线粒体DNA缺失对人乳腺癌细胞药物敏感性的影响。方法 采用溴化乙锭（EB）处理获得人乳腺癌细胞MDA—MB-231的线粒体DNA缺失（rho^0 MDA—MB-231）细胞，电镜下观察细胞形态改变，胶原凝胶包埋三维立体培养法（CD—DST）对比分析细胞的药物敏感性，免疫组织化学方法检测P-糖蛋白（P—gP）、乳腺癌耐药蛋白（BCRP）的表达。结果 与MDA—MB-231细胞比较，rho^0 MDA—MB-231细胞线粒体数量增多，嵴消失呈空泡样改变，对化疗药物相对抵抗，P—gP、BCRP的表达明显增加。结论 线粒体的损伤可能参与了乳腺癌细胞耐药表型的形成。

Effect of mitochondrial DNA deletion on the sensitivity of MDA-MB-231 cells to chemotherapeutic drugs

Objective To explore the relationship between mitochondrial DNA deletion and drug resistance in human breast cancer cells.Methods The rho0-derivative of MDA-MB-231 was generated by treating the cultured cells with ethidium bromide.The morphologies were confirmed by transmission electron microscope(TEM).The sensitivities to chemotherapy were determined by Collagen Gel Droplet Embedded Culture-Drug Sensitivity Test(CD-DST).P-glycoprotein(P-gp)and breast cancer resistance protein(BCRP)expression were detected ...