| 人神经营养素-3全长基因克隆及序列分析 |
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| 引用本文： | 陈传好,陈昌杰,赵莉.人神经营养素-3全长基因克隆及序列分析[J].蚌埠医学院学报,2005,30(1):9-11. |
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| 作者姓名： | 陈传好 陈昌杰 赵莉 |
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| 作者单位： | 1. 蚌埠医学院人体解剖学教研室, 安徽蚌埠 233003;2. 蚌埠医学院生物化学与分子生物学教研室, 安徽蚌埠 233003 |
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| 基金项目： | 安徽省教育厅优秀青年科研项目 |
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| 摘 要： | 目的: 扩增人神经营养素-3(human neurotrophin-3,hNT-3)全长基因并进行序列分析。方法: 应用聚合酶链反应技术,以一个健康成人基因组DNA为模板,扩增出编码hNT-3的全长基因,并克隆至pMD18-T载体中进行基因测序。结果: 所得序列与Genbank提供的序列(M61180)对比显示,除hNT-3中第555位碱基由C→T外,余序列同已知序列完全一致,改变的碱基并不影响基因编码的氨基酸序列。结论: 成功地获得了hNT-3的全长基因,且序列与已知序列高度一致。
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| 关 键 词： | 神经生长因子 神经营养素-3 基因克隆 聚合酶链反应 DNA序列分析 |
| 文章编号： | 1000-2200(2005)01-0009-03 |
| 收稿时间： | 2004-02-04 |
Cloning and sequencing of human neurotrophin-3 intact gene |
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| CHEN Chuan-hao ,CHEN Chang-jie ,ZHAO Li.Cloning and sequencing of human neurotrophin-3 intact gene[J].Journal of Bengbu Medical College,2005,30(1):9-11. |
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| Authors: | CHEN Chuan-hao CHEN Chang-jie ZHAO Li |
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| Institution: | 1. Department of Anatomy, Bengbu Medical College, Anhui 233003, China;2. Department of Biochemistry and Molecular Biology, Bengbu Medical College, Anhui 233003, China |
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| Abstract: | Objective:To amplify and sequence the human neurotrophin-3(hNT-3) intact gene.Methods:hNT-3 intact gene was obtained by polymerase chain reaction using human genomic DNA as a template and cloned into pMD18-T vector and sequenced.Results:DNA sequence comparison of the cloned fragments and the reported hNT-3(M61180) demonstrated that the gene sequence was identical to that had been reported before except one base(555th) changing from C to T,but it had no influence on the encoded amino acids.Conclusions:hNT-3 intact gene was obtained and its sequence was highly consistent with ones reported. |
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| Keywords: | nerve growth factor neurotrophin-3 gene cloning PCR DNA sequencing |
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