子宫内膜异位症患者腹腔液中自然杀伤细胞活性及前列腺素和白介素-6水平的研究

目的探讨子宫内膜异位症(EM)患者的腹腔液(PF)中白细胞介素-6(IL-6)、前列腺素(PGs)的含量变化与PF对自然杀伤细胞(NK)活性影响的关系。方法选择42例EM患者作为观察组(EM组),13例症状相似的非EM患者作为对照组,采用乳酸脱氢酶(LDH)释放法测定NK细胞活性。放射免疫法测定PF中PGs和IL-6的含量。结果作用2h时,非EM患者和EM患者的PF对NK细胞活性的抑制率分别为34.8%±26.2%和66.1%±30.4%,两者比较有显著性差异(P<0.01)。EM组抑制率与EM临床分期呈正相关(r=0.6207,P<0.01)。EMⅠ~Ⅱ期患者PF的IL-6含量为0.62±0.45μg/L,Ⅲ~Ⅳ期患者为0.48±0.35μg/L,对照组为0.41±0.38μg/L,三者间差异无显著性(P>0.05)。PF中IL-6的含量与其对NK细胞活性的抑制率之间无显著相关(r=0.2231,P>0.05)。EM患者PF中PGE2和PGF2ɑ含量均较对照组明显升高(P均<0.01),其中Ⅰ、Ⅱ期患者PF对NK细胞抑制率与PGE2、PGF2ɑ含量呈正相关(r=0.5098或0.4102,P均<0.01)。结论EM患者的PF对NK细胞活性具有明显的抑制作用,Ⅰ、Ⅱ期患者PF中PGs的增高可能对NK细胞活性有一定的抑制作用。

Analysis of the activation of NK cell and the levels of prostaglandins and interleukin-6 in endometriosis women''''s peritoneal fluid

Objective To investigate the level of prostaglandins(PGs) and interleukin-6(IL-6) in the peritoneal fluid(PF) of the women with endometriosis(EM), and the relations of PF to the activation of NK cell . Methods There were 42 cases of EM and 12 cases of non- EM with similar symptoms. The LDH releasing assay was used to measure the activity of NK cell. The concentration of IL-6 and PGE_2 were measured by radioimmunoassay. Results After two hours,the inhibition rate of NK cell activity in EM and non-EM decreased by 34.8%±26.2% and 66.1%±30.4%, respectively. The difference was significant statistically (P<0.01). The inhibition rate had positive correlation with the classifications of EM(r=0.6207,P<0.01). The concentration of IL-6 in PF was 0.62±0.45 μg/L in EM TypeⅠ~Ⅱ, 0.48±0.35 μg/L in EM TypeⅢ~Ⅳ, and 0.41±0.38 μg/L in non-EM. There was no significant statistically among them(P>0.05). The level of interleukin-6 in PF had no relation with the inhibition rate of NK cell activity. The concentration of PGE_2 and PGF_2, both increased significantly in EM,which correlated especially with the suppressive effect of NK activity in EM TypeⅠ~Ⅱ.Conclusion PF of EM can significantly decrease NK cell activity in vitro. The increasing concentration of PGs in PF of EM TypeⅠ~Ⅱ may inhibit activity of the NK. 【