抑肽酶对心脏体外循环术后TNF—α水平的影响

采用双抗体夹心酶联免疫吸附法测定不同剂量抑肽酶对心脏体外循环（ＣＰＢ）术中肿瘤坏死因子－α（ＴＮＦ－α）的含量。结果显示：ＣＢＰ术后２４ｈ血浆ＴＮＦ－α含量，在全量抑肽酶组较对照组有明显降低（Ｐ〈０．０５）；在单次剂量抑肽酶组较对照组无差异（Ｐ〈０．０５）。表明全量抑肽酶具有抗炎效果。

Effects of aprotinin on TNF-alpha levels after cardiopulmonary bypass]

OBJECTIVE: This study evaluates the anti-inflammatory activity of full-dose and pump-prime-only(low-dose) aprotinin by means of comparing tumor necrosis factor alpha(TNF-alpha) levels of both groups after CPB. METHODS: Twenty-nine adults with rheumatic heart disease were randomized into three groups: (1) full-dose aprotinin-treated group(Group A, n = 10); (2) pump-prime-only aprotinin-treated group(Group B, n = 10), and (3) control group(Group C, n = 9). Plasma concentrations of TNF-alpha were measured by enzyme-linked immunosorbent assay technique at baseline(before operation), and at 2, 24 hours after CPB termination. RESULTS: A significant(P < 0.05) increase of TNF-alpha occurred in all three groups at 2 and 24 hours after CPB termination when compared with the same group at baseline. In Group A, TNF-alpha level was significantly lower than that in group C(P < 0.05) at 24 hours after CPB, but not in group B(P > 0.05). CONCLUSIONS: Both CPB and operative stimulus induce the increase of cytokine TNF-alpha after CPB. Full-dose aprotinin has the anti-inflammatory effect by means of reducing TNF-alpha level after CPB. Low-dose aprotinin dose not reduce TNF-alpha level. So it no significant anti-inflammatory effect.