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| 原位杂交技术鉴定马尔尼菲青霉 | |
| 引用本文： | 曹存巍,邓卓霖,罗虹,李佳全,韦义萍. 原位杂交技术鉴定马尔尼菲青霉[J]. 中国皮肤性病学杂志, 2004, 18(8): 462-463 |
| 作者姓名： | 曹存巍 邓卓霖 罗虹 李佳全 韦义萍 |
| 作者单位： | 1. 广西医科大学第一附属医院皮肤科,广西,南宁,530021 2. 广西医科大学第一附属医院病理科,广西,南宁,530021 3. 广西医科大学第一附属医院医学实验中心,广西,南宁,530021 4. 广西医科大学第一附属医院护理学院,广西,南宁,530021 |
| 摘 要： | 目的 探讨原位杂交技术 (ISH)特异性检测马尔尼菲青霉 (Pm)感染组织标本的可行性。方法 以Pm的rDNA区间种特异性序列作为特异性探针 ,通过聚合酶链反应 (PCR)扩增合成并以地高辛标记 ,与 12例临床确诊的Pm感染组织标本中的靶DNA杂交。结果 12例Pm感染标本中全部阳性 ,其它种属真菌感染组织均为阴性。结论 ISH技术可以灵敏、特异地检测出组织中的Pm ,适用于今后临床组织标本中Pm感染的检测。 |
| 关 键 词： | 马尔尼菲青霉 原位杂交 DNA探针 |
| 文章编号： | 1001-7089(2004)08-0462-02 |
Identification of Penicillium Marneffei by in Situ Hybridization Technique |
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| CAO Cun-wei,DENG Zhuo-lin,LUO Hong,et al. Identification of Penicillium Marneffei by in Situ Hybridization Technique[J]. The Chinese Journal of Dermatovenereology, 2004, 18(8): 462-463 | |
| Authors: | CAO Cun-wei DENG Zhuo-lin LUO Hong et al |
| Abstract: | Objective To evaluate the applicability of in situ hybridization (ISH) technique in the specific diagnosis of clinical tissue specimens infected with penicillium marneffei(Pm).Methods Twelve formalin-fixed and paraffin-embeded tissue specimens from patients with Pm infected were studied by an ISH technique, The specific probe was selected from a Pm rDNA region, synthesized and digoxigenin labeled by means of polymerase chain reaction (PCR).Results Positive staining of Pm was observed in all 12 tissue specimens infected with Pm,but none of positive staining occurring in other genus or species fungi had been found.Conclusion The ISH is a sensitive and specific technique for identification of Pm infection, It could be applicability to clinical molecular diagnosis of Pm infection. |
| Keywords: | Penicillium marneffei In situ hybrdization DNA probe. |
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