腹腔开放伤并海水浸泡对犬血浆自由基水平的影响

目的：了解腹腔开放伤后海水浸泡对机体血浆中自由基水平的影响。方法：28只犬随机均分为对照组（A组），单纯腹部浸泡组（B组），腹腔等渗盐水浸泡组（C组）和腹腔海水浸泡组（D组）4个组。A组为单纯腹外伤，B组为直接将犬无伤腹部浸入海水中浸泡处理，C，D两组犬致伤后分别置入等渗盐水或海水中浸泡处理，观察血浆中丙二醛（malondialdehyde,MDA)，超氧化物歧化酶（superoxide dismutase,SOD)及血浆渗透压的变化。结果：犬腹腔开放伤并海水浸泡后60min时，血浆中MDA与血浆渗透压水平显著升高（与浸泡前及A，B，C，组比较，P＜0．01），并随浸泡时间延长呈升高趋势，而同时SOD水平则显著降低（与浸泡前及A，B，C组比较，P＜0．01），并随浸泡时间延长呈降低趋势；浸泡处理前后A，B，C组间差别未见有显著性（P＞0．05），结论：腹腔开放伤区海水浸泡可引起机体自由基水平升高。

Effect of seawater immersion on plasma MDA and SOD levels in dogs with open celiac wound

Objective. To determine the effect of seawater immersion on plasma levels of free radicals in dogs with open celiac wound. Methods Twenty ?eight dogs were randomly divided into four groups; control group (group A),simple abdominal seawater immersion group (group B),celiac iso-tonic saline immersion group (group Oand celiac seawater immersion group (group D). Group A was with only open celiac wound. The abdomens of group B was immersed with seawater directly. The abdomens of group C and D were immersed with isotonic. saline or seawater respectively after open celiac wound. Indexes as malondialdehyde (MDA) ,superoxide dismutase(SOD) and plasma osmotic pressure were observed. Results The plasma levels of MDA and osmotic pressure in group D were increased significantly 60 min after seawater immersion (compared with preoperation and group A,B or C,.P0. 05). Conclusions Seawater immersion could increase the levels of oxygen free radicals in dogs with open celiac wound.