Mapping of the RIB5 gene in Saccharomyces cerevisiae using UV light as an enhancer of rad52-mediated chromosome loss |
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Authors: | María de los Angeles Santos Enrique A. Iturriaga Arturo P. Eslava |
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Affiliation: | (1) Departamento de Microbiología, Genética, Medicina Preventiva y Salud Pública, Universidad de Salamanca, E-37008 Salamanca, Spain |
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Abstract: | Summary Ribs mutants of S. cerevisiae are blocked at the end of the riboflavin biosynthetic pathway. Using UV light to increase rad52-mediated chromosome loss, we have assigned the rib5 mutation to chromosome II. Tetrad analysis of crosses between rib5 and other markers on chromosome II shows that the RIB5 gene is located on the right arm of this chromosome, closely linked to HIS7. |
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Keywords: | S. cerevisiae rad52-chromosome loss UV light Riboflavin |
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