缺血再灌注损伤降低肠黏膜屏障功能的初步研究

目的 ]观察缺血再灌注损伤对肠黏膜屏障功能的影响。 方法 ]将 1 0只昆明种小鼠和 1 6只SD大鼠随机分为对照组和缺血再灌注组 ,小鼠每组 5只 ,大鼠每组 8只。取小鼠空肠和结肠肠壁组织 ,制备组织切片 ,观察光镜及电镜下肠黏膜形态学变化 ;取大鼠肠系膜前淋巴结 ,细菌培养 4 8h后观察细菌易位变化。 结果 ]光镜下观察到缺血再灌组空肠肠壁变薄 ,绒毛短细 ,绒毛间距变宽 ,局部黏膜上皮坏死脱落 ,固有层充血淤血 ,结肠变化不明显 ;电镜下缺血再灌组与对照组相比未见明显改变 ;缺血再灌组细菌易位率为 5 / 8,对照组为 1 / 8(P <0 .0 5 )。 结论 ]缺血再灌注对小鼠和大鼠肠黏膜机械和免疫屏障有一定程度的损伤。

Decrease of intestinal barrier after ischemia-reperfusion injury in animals

To observe the changes of intestinal barrier after ischemia-reperfusion injury (I/R) in mice and rats. 10 mice of Kunming species and 16 rats of SD species were divided randomly into 2 groups: control group and I/R group. There are 5 mice and 8 rats in each group. The mice's jejumum and colon were exenterated and made into slides, and their morphological changes were observed under light and electrical microscopes. The bacterial translocation rates of mesenteric lymph nods(MLN) were detected after 48 hours culture of rats' MLN. Compared to control group, obvious morphological changes were observed in jejunum, but not in colon, under LM in I/R group: the intestinal wall became thinner, the intestinal villi grew shorter and the interspace between the villi became wider, and parts of the intestinal villi underwent necrosis and broke off, also there were congestion and hemorrhage in lamina propria. No distinct changes of tight junction were observed under EM in jejunum and colon after I/R. The bacterial translocation rates of MLN is 5/8 in I/R group and 1/8 in control group( P <0.05). Conclusion] Ischemia-reperfusion injury induces intestinal mucosal damage and bacterial translocation in mice and rats.