新城疫病毒D817对裸小鼠人结肠癌移植瘤的抑制作用

目的 观察新城疫病毒DK/HK/817/1980(NDV D817)对裸小鼠人结肠癌移植瘤的抑制作用.方法 采用LoVo细胞株建立裸小鼠人结肠癌移植瘤模型,分别给予裸小鼠尾静脉注射PBS、氟尿嘧啶(5-Fu)以及NDV D817高、中、低3个剂量,观察不同剂量NDV D817对移植瘤的抑制作用,病理学观察NDV D817对移植瘤和肝细胞的损伤程度,流式细胞术检测肿瘤细胞的凋亡和坏死情况,酶联免疫吸附试验(ELISA)试剂盒检测荷瘤小鼠体内肿瘤坏死因子-α(TNF-α)的含量,血凝实验检测荷瘤小鼠体内活病毒量.结果 中剂量NDV D817可明显抑制移植瘤的生长,肿瘤抑制率达48.1%.NDV D817对荷瘤小鼠体重和肝脏的损伤较小,且具有诱导肿瘤细胞凋亡和诱导机体产生TNF-α的能力.荷瘤小鼠处死后只在瘤内检测到活病毒,而在血清和其他脏器中并没有检出活病毒.结论 在对裸小鼠人结肠癌移植瘤的抑制过程中,NDV D817有明显的抑瘤效果,适当剂量的NDVD817更安全有效.

Anti-tumor effect of Newcastle disease virus strain D617 against nude mouse xenografts of human colon carcinoma

Objective To study the anti-tumor effects of Newcastle disease virus (NDV) strain D817 on human colon carcinoma model in nude mice. Methods The nude mouse model of human colon carcinoma was established by subcutaneous inoculation of human colon cancer LOVO cells. The tumor-bearing mice were given PBS, 5-Fu, high-dose NDV D817, moderate-dese NDV D817 or low-dose NDV D817 via caudal vein injection. The tumor size and weight of mice were measured. The liver damages were examined by histopathology. Apoptosis and necrosis of tumor ceils were detected by flow cytometry. The endotumoral content of TNF-α was detected using a mouse TNF-a ELISA kit. The live vires was detected by bemagglutination (HA) test. Results The moderate-dose NDV D817 inhibited the tumor growth more apparently than 5-Fu. The tumor growth inhibition rate reached to 48.1%. The liver damage and the weight change caused by NDV were less severe. NDV D817 made an increased apoptosis index and induced production of TNF-α. Live virus was not detected in important organs except in the tumor of nude mice by HA test. Conclusion In the anti-tumor process in nude mice bearing xenografts of human colon carcinoma, a suitable dose of NDV D817 is more safe and effective.