| 5-FU有机金属框架纳米粒的体外细胞摄取及影响因素的研究 |
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| 引用本文： | 李秀岩,李英鹏,王艳宏,王子涵,辛丽丽,李永吉.5-FU有机金属框架纳米粒的体外细胞摄取及影响因素的研究[J].华西药学杂志,2017,32(2). |
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| 作者姓名： | 李秀岩 李英鹏 王艳宏 王子涵 辛丽丽 李永吉 |
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| 作者单位： | 黑龙江中医药大学药学院,黑龙江哈尔滨,150040 |
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| 基金项目： | 黑龙江中医药大学科研基金 |
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| 摘 要： | 目的 制备5-氟尿嘧啶(5-FU)有机金属框架(BTC)纳米粒,考察体外细胞摄取率及其影响因素.方法 用室温配位调控法制备BTC纳米粒5-FU-Cu-BTC,用扫描电子显微镜观察其形态,用流式细胞仪测定其体外细胞摄取率及影响因素.结果 Cu-BTC载体的粒径约为100 nm,载药量为38％,体外细胞摄取实验表明:细胞摄取率与样品浓度和温度成正比.结论 有机金属框架纳米粒可载入抗肿瘤药物并被肿瘤细胞摄取.
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| 关 键 词： | 纳米有机金属框架 5-氟尿嘧啶 Cu-有机金属框架 体外细胞摄取 纳米粒 流式细胞仪 |
Preparation of 5-FU nanosized metal-organic framework and evaluation of their cell specificity |
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| LI Xiuyan,LI Yingpeng,WANG Yanhong,WANG Zihan,XIN Lili,LI Yongji.Preparation of 5-FU nanosized metal-organic framework and evaluation of their cell specificity[J].West China Journal of Pharmaceutical Sciences,2017,32(2). |
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| Authors: | LI Xiuyan LI Yingpeng WANG Yanhong WANG Zihan XIN Lili LI Yongji |
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| Abstract: | OBJECTIVE To prepare 5-FU nanosized metal-organic framework and to evaluate their cell specificity and the influence factors.METHODS 5-FU nanosized metal-organic framework (5-FU-Cu-BTC) were prepared by coordination control method at room temperature.The mophorlogy of 5-FU-Cu-BTC was observed by scanning electron microscopy.Flow cytometer (FCM) was performed to evaluate the cellular uptake and their influence factors.RESULTS The mean size of Cu-BTC was about 100 nm.The drug loading capacity was 38％.The cellular uptake experiment reveals that cellular uptake rate was proportional to the sample concentration and the incubation temperature.CONCLUSION Metal-organic framework nanoparticles is able to deliver antitumor drugs into tumor cells. |
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| Keywords: | Nanosized metal-organic framework 5-FU Cu-BTC Cellular uptake in vitro Nanoparticles Flow cytometer |
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