基于Bcl-2蛋白关键位点抑制剂的研究

目的 设计并合成高抑制活性的小分子Bcl-2蛋白抑制剂.方法 基于已报道的活性小分子,运用Autodock 4.2软件研究其与Bcl-2蛋白的作用模式,探索Bcl-2蛋白的关键位点并设计、合成一系列新型小分子抑制剂,并进行体外抗肿瘤活性的实验.结果 设计合成了8个全新的小分子化合物,其结构经1HNMR和13CNMR确证.结论 所合成的8个化合物均有明显的体外抗肿瘤活性,且大部分高于阳性对照紫杉醇.

Study of inhibitors based on the key sites of Bcl-2 protein

OBJECTIVE To design and synthesize Bcl-2 protein inhibitors of high inhibitory activity.METHODS Based on the active molecules which had been reported,the mode of action with the Bcl-2 protein were researched using Autodock 4.2,and a series of new molecule inhibitors were designed and synthesized.The in vitro anti-tumor activity experiments were studied on Hela,B16,C26 and SW620 cells by MTT method.RESULTS Eight kinds of new small Bcl-2 protein inhibitors were synthesized.The structures of the compounds had been determined by 1HNMR and 13CNMR.CONCLUSION The new compounds process good anti-tumor activity,and the activity is much higher than the positive control drug.