| HPLC测定益肝除湿合剂中的没食子酸 |
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| 引用本文： | 王育,陈勇,杨胜玉. HPLC测定益肝除湿合剂中的没食子酸[J]. 华西药学杂志, 2017, 32(5). DOI: 10.13375/j.cnki.wcjps.2017.05.028 |
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| 作者姓名： | 王育 陈勇 杨胜玉 |
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| 作者单位： | 1. 四川省达州市食品药品检验所,四川达州,635099;2. 成都地奥集团天府药业股份有限公司,四川达州,635027 |
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| 摘 要： | 目的 采用HPLC法测定益肝除湿合剂中没食子酸的含量.方法 采用Diamonsil C18色谱柱(250 mm×4.6 mm,5μm),以甲醇-乙腈-0.1％磷酸溶液(5∶6∶89)为流动相,检测波长264.5 nm,流速1 mL· min-1,柱温30℃.结果 0.01～2.54 μg没食子酸与峰面积的线性关系良好(r=0.9997),平均回收率为99.32％,RSD =0.90％(n=6).结论 所用方法简便、易控、准确可靠,可用于益肝除湿合剂的质量控制.
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| 关 键 词： | 益肝除湿合剂 高效液相色谱法 没食子酸 含量测定 |
Determination of gallic acid in Yigan Chushi mixture by HPLC |
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| WANG Yu,CHEN Yong,YANG Shengyu. Determination of gallic acid in Yigan Chushi mixture by HPLC[J]. West China Journal of Pharmaceutical Sciences, 2017, 32(5). DOI: 10.13375/j.cnki.wcjps.2017.05.028 |
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| Authors: | WANG Yu CHEN Yong YANG Shengyu |
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| Abstract: | OBJECTIVE To establish an HPLC method for the determination of gallic acid in Yigan Chushi mixture (YCM) by HPLC.METHODS The Diamonsil-C18 column was used (250 mm × 4.6 mm,5 μm).The mobile phase was methanol-acetonitrile-0.1％ Phosphoric acid (5 ∶ 6 ∶ 89).The flow rate was 1.0 mL·min-1.The column temperature was at 30 ℃.The detection wavelength was 264.5 nm.RESULTS The linear range of gallic acid was 0.01-2.54 μg (r =0.9997).The average recovery of sample was 99.32％ with the RSD of 0.90％ (n =6).CONCLUSION The method is simple,easy to control,accurate and reliable,and can be used for the quality control of YCM. |
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| Keywords: | Yigan Chushi mixture HPLC Gallic acid Determination |
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