一清胶囊中7种指标成分的测定

目的 采用反相高效液相色谱法同时分离测定一清胶囊中7种指标成分的含量.方法 采用Kromasil ODS-C18色谱柱(200 mm×4.6 mm,5μm)进行梯度洗脱,流动相A为0.5％三乙胺-甲醇(95∶5,冰乙酸调pH3.12),流动相B为0.5％三乙胺-甲醇(5∶95,冰乙酸调pH4.00),检测波长254 nm,流速1.0 mL· min-.结果 一清胶囊中7种指标成分的线性关系良好,r为0.9997 ～0.9999,平均回收率为96.18％～101.71％,RSD均符合要求.结论 所用方法的测定结果稳定、可靠、准确、专属性强,可用于一清胶囊的质量控制.

Determination of the seven marker components in Yiqing capsule

OBJECTIVE To simultaneously determine the seven marker components in Yiqing capsule (YQC) by RP-HPLC.METHODS The gradient elution was executed on a Kromasil C18 column (200 mm × 4.6 mm,5 μm) at flow rate of 1.0 mL·min-1.The mobile phase A consisted of 0.5％ triethylamine and methanol(95 ∶ 5,adjusting pH to 3.12 with glacial acetic acid),and the mobile phase B consisted of 0.5％ triethylamine and methanol(5 ∶ 95,adjusting pH to 4.00 with glacial acetic acid).The detection wavelength was at 254 nm.The column temperature was maintained at 25 ℃.RESULTS The seven marker ingredients in YQC had good linearities and r ranged from 0.9997 to 0.9999.The average recoveries of the seven marker components were 96.18％-101.71％.RSDs met the requirements of assay.CONCLUSION This method is reliable,accurate and specific.It can be used to control the quality of YQC.