蜕皮甾酮对HepG2细胞葡萄糖消耗的影响

目的探讨蜕皮甾酮体外降糖的作用特点或机制,即能否通过刺激胰岛素分泌而产生降糖作用。方法检测HepG2细胞24 h培养液中葡萄糖的消耗量;另外测定βTC3细胞胰岛素的释放量。结果蜕皮甾酮在1×10-6~10-4mol.L-1浓度范围内可使HepG2细胞的葡萄糖消耗量增加(44%~77%);蜕皮甾酮的降糖效能随着培养液中葡萄糖浓度的升高而降低;胰岛素对蜕皮甾酮的降糖作用没有明显的影响。蜕皮甾酮没有刺激βTC3细胞胰岛素分泌的作用。结论蜕皮甾酮可通过肝细胞发挥非胰岛素依赖的降糖作用,但不能刺激胰岛素的分泌。

Effect of ecdysterone on glucose consumption of HepG2 Cells

Aim To investigate whether ecdysterone is able to exert glucose-lowering effect on hepatocytes or stimulate the secretion of insulin.Methods For glucose consumption studies,the amounts of glucose disappeared from the culture medium of HepG2 cells within 24 h were determined.βTC3 cells were also used to monitor insulin secretion.Results The glucose concentrations decreased significantly by ecdysterone 1×10~(-6)～10~(-4) mol·L~(-1),while glucose consumption increased by 44%～77% with ecdysterone.Glucose consumption declined as its concentrations increased.Insulin had no effect on glucose-lowering of ecdysterone.βTC3 cells were not stimulated by ecdysterone.Conclusion Ecdysterone is able to exert glucose-lowering effect on hepatocytes which is insulin independent,but has no effect on insulin secretion.