| 糖尿病肾阴虚证模型大鼠尿液的代谢组学研究 |
| |
| 引用本文： | 孙永宁,刘忠,冯雯. 糖尿病肾阴虚证模型大鼠尿液的代谢组学研究[J]. 成都中医药大学学报, 2009, 32(2): 69-71 |
| |
| 作者姓名： | 孙永宁 刘忠 冯雯 |
| |
| 作者单位： | 上海交通大学附属第六人民医院,上海,200233;上海交通大学药学院,上海,200300 |
| |
| 基金项目： | 上海交通大学医工(理)交叉研究基金 |
| |
| 摘 要： | 目的:研究糖尿病肾阴虚证大鼠模型尿液的代谢表型变化,探讨代谢组学在糖尿病肾阴虚证研究中的应用.方法:将大鼠分为正常组、模型组,模型组采用甲状腺素和链脲佐茵素分别皮下和腹腔注射的方法来制作糖尿病阴虚证大鼠模型;收集造模组大鼠的尿液,进行GC-MS检测.结果:模型组大鼠血糖增高,尿糖、尿蛋白均增高.与正常组比较,模型组大鼠的尿液中包含的甘氨酸、琥珀酸含量显著升高(P<0.05),十六烷酸、胆固醇含量显著下降(P<0.05).结论:大鼠的尿液代谢谱与糖尿病肾阴虚证密切相关,代谢组学在中医糖尿病研究中有广泛前景.
|
| 关 键 词： | 糖尿病 肾阴虚证 动物模型 代谢组学 GC-MS |
ESSENTIAL EMPIRICAL STUDY OF THE DEFICIENCY OF KIDNEY-YIN IN DIABETES MELLITUS BASED ON METABONOMICS |
| |
| SUN Yong-ning,Liu Zhong,FENG Wen. ESSENTIAL EMPIRICAL STUDY OF THE DEFICIENCY OF KIDNEY-YIN IN DIABETES MELLITUS BASED ON METABONOMICS[J]. Journal of Chengdu University of Traditional Chinese Medicine, 2009, 32(2): 69-71 |
| |
| Authors: | SUN Yong-ning Liu Zhong FENG Wen |
| |
| Abstract: | Objective: Research the deficiency of kidney-YIN in diabetes mellitus rat models' urine based on GC-MS technology.Methods: The rats were divided into normal group,model group.Administered streptozotocin hypodermic injection and then administered Streptozocin intraperitoneal injection to establish model in Wistar rats.Collected the rats' urine and detected the composition and the contents of the samples by the GC-MS spectrogram.Results: The rats' FBG were all above 11.1mmol/L.The rats' urine glucose and urin... |
| |
| Keywords: | GC-MS |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
