精子功能检测的研究——Ⅰ.底物膜技术对精子顶体酶的测定

本研究应用健康人和不育症患者的精液各30人份,用底物膜技术检测精子顶体酶,同时进行精子形态学和多次曝光摄片分析。结果表明,在精子头部周围,顶体蛋白酶和透明质酸酶活性区域呈现亮区或晕。不育症患者顶体蛋白酶反应率和反应区的平均直径显著降低(P<0.01),但是透明质酸酶无明显变化(P>0.05).不育症患者精子畸形率高于健康人.不育症患者和健康人精子运动平均速度相似,但直线运动方式的精子在前者明显减少.本文对精子的顶体酶、形态异常和运动等相互关系作了讨论.

STUDY ON THE ASSESSMENT OF SPERM FUNCTION I. DETERMINATION OF SPERM ACROSOMAL ENZYMES BY SUBSTRATE FILM TECHNIQUE

In this study, thirty fertile and infertile semen sample were used. The acrosomal enzymes of spermatozoa were demonstrated by using substrate film method together with morphological analysis and MEP, The results showed that the site of protease or hyaluronidase activity appeared around the sperm head as clear areas or halos. In infertile men, the percentage of reaction and the average diameter of reaction area of the protease were decreased significantly (p<0.01), but the hyaluronidase did not show marked changes (p<0.05). The rate of abnormal sperm of infertile men was higher than that of fertile men. The average velocity of sperm movement was similar in the infertile and fertile men, but the percentage of forward progression spermatozoa was much lower in the former. The relation between acrosomal enzymes, abnormal morphology and sperm motility was discussed.