阿托伐他汀对自发性高血压大鼠HMG-CoA还原酶表达的影响

 目的：评价阿托伐他汀对自发性高血压大鼠（SHR）HMG-CoA还原酶表达的影响。 方法：12只8周龄的SHR随机分为蒸馏水饲养组（SHR_DW组，n＝6）与阿托伐他汀治疗组（SHR_ATV组，n＝6），并以6只同周龄的正常血压大鼠（WKY）作为对照（WKY组，n＝6）。采用RT-PCR与Western blotting法分别检测HMG-CoA还原酶的mRNA及蛋白表达。同时检测血压与血脂。 结果：给药10周后，SHR_ATV组收缩压显著低于治疗前及SHR_DW组（P<0.05），其血清TC、TG、LDL-C及HDL-C的水平与SHR_DW组及WKY组相比，也明显降低（P<0.05）；SHR_ATV组HMG-CoA还原酶mRNA的表达水平在给药10周后显著低于WKY组及SHR_DW组（P<0.05），其蛋白表达水平同样出现类似的结果。 结论：阿托伐他汀能够下调HMG-CoA还原酶的mRNA及蛋白表达水平，不仅使SHR的血脂降低，在某种程度上，还可能与其血压的下降有关。

Effect of atorvastatin on HMG-CoA reductase expression in spontaneously hypertensive rats

AIM:To evaluate the effect of atorvastatin on HMG-CoA reductase(HMGR)expression level in spontaneously hypertensive rats(SHR).METHODS:Twelve eight-week-old SHR was randomized into distilled water group(SHR_ DW group,n=6)and atorvastatin-treated group(SHR_ ATV group,n=6).The age-matched Wistar-Kyoto rats(WKY)were used as control(WKY group,n=6).RT-PCR and Western blotting were used to detect HMGR mRNA and protein expression levels,respectively.Meanwhile,systolic blood pressure(SBP)and serum lipid levels were examined.RESULTS:Ten weeks a treatment,SBP in SHR_ ATV group was markedly decreased compared with before treatment and SHR_ DW group(P<0.05).The levels of TC,TG,LDL-C and HDL-C in SHR_ ATV group were also markedly lower than those in SHR_ DW group and WKYgroup(P<0.05).10 weeks later,HMGR mRNA levels decreased markedly in SHR_ ATV group compared to WKY group and SHR_ DW group(P<0.05).The similar results were found in HMGR protein expression levels.CONCLUSIONS:Atorvastatin downregulates mRNA and protein expression levels of HMGR in SHR,which not only decreases the serum lipids levels,but also impacts blood pressure to a certain extent.