同种异体半月板联合骨软骨移植的实验研究

目的:探讨新鲜同种异体半月板骨软骨联合移植治疗胫骨平台毁损伤后骨关节炎的疗效。方法:成年新西兰大白兔36只,随机分为A、B、C3组,各12只。A组行右膝内侧半月板连同胫骨平台骨软骨移植,克氏针交叉固定骨块。B组行右膝内侧半月板移植,左膝内侧半月板取出制备新鲜冷冻半月板。C组行左膝内侧新鲜冷冻半月板移植。术后4、8、12周分批取材行大体观察、组织学检查和胫骨平台软骨氨基己糖(GAG)测定。结果:12周时A组移植胫骨平台软骨与B、C组半月板移植术后的内侧胫骨平台软骨氨基己糖含量差异无统计学意义;A、B组移植的半月板纤维软骨细胞数差异无统计学意义;A组半月板移植的纤维软骨细胞数多于C组。结论:新鲜同种异体半月板骨软骨联合移植能修复胫骨平台毁损伤。

Experimental study on fresh meniscal allografts combined with osteochondral allografts transplantation

Objective:To investigate the effect of fresh meniscal allografts combined with osteochondral allografts transplantation for treatment of osteoarthritis. Methods:Thirty-six rabbits were used in the experiment and were randomly divided into 3 groups:in group A,the fresh medial meniscal allografts combined with osteochondral allografts from medial tibial plateau were implanted into medial articular meniscal and medial tibial plateau osteochondral defects;in group B,the fresh medial meniscal allografts were implanted into medial meniscal allografts defects;in group C,the freezing medial meniscal allografts were implanted into medial meniscal allografts defects. General observation,histology examination and glycosaminoglycan (GAG) examination in cartilage of medial tibial plateau were performed at the 4th,8th and 12th week after operation. Results:There were no significant differences in cellular counting and amount of GAG between group A and group B,but the cellular amount of group A was significantly more than that of group C at the 12th week. Conclusion:Fresh meniscal allografts combined with osteochondral allografts transplantation can repair meniscal and osteochondral defects.