| α_1-抗糜蛋白酶基因Bochum-1、Bonn-1突变型与儿童哮喘相关性研究 |
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| 引用本文： | 黄锐,曹虹,李海粟,罗放灵.α_1-抗糜蛋白酶基因Bochum-1、Bonn-1突变型与儿童哮喘相关性研究[J].中国优生与遗传杂志,2010(4):21-23. |
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| 作者姓名： | 黄锐 曹虹 李海粟 罗放灵 |
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| 作者单位： | 昆明市延安医院儿科;昆明市延安医院检验科; |
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| 摘 要： | 目的了解α1-抗糜蛋白酶（AACT）基因突变与昆明儿童哮喘发生是否具有相关性。方法应用聚合酶链反应（PCR）技术、限制性片段长度多态性（RFLP）分析方法,选择昆明市汉族哮喘儿童及正常儿童各50例,对α1-抗糜蛋白酶基因外显子（exon）Ⅱ、Ⅲ进行检测。结果所有研究对象均未发现α1-抗糜蛋白酶基因Bochum-1、Bonn-1突变型。结论α1-抗糜蛋白酶基因Bochum-1、Bonn-1突变型与50例昆明市汉族儿童哮喘发病无相关关系。
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| 关 键 词： | 儿童哮喘 α1-抗糜蛋白酶 基因突变 聚合酶链反应 |
Study on α_1-antichymotrypsin gene Bochum-1,Bonn-1 mutations among asthmatic children |
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| HUANG Rui,CAO Hong,LI Hai-su,LUO Fang-ling..Study on α_1-antichymotrypsin gene Bochum-1,Bonn-1 mutations among asthmatic children[J].Chinese Journal of Birth Health & Heredity,2010(4):21-23. |
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| Authors: | HUANG Rui CAO Hong LI Hai-su LUO Fang-ling |
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| Institution: | HUANG Rui,CAO Hong,LI Hai-su,LUO Fang-ling.(Department of Pediatrics,Yan\'an Hospital,Kunming 650051) |
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| Abstract: | Objective：To understand α1-antichymotrypsin gene mutations of children asthma in Kunming.Methods：50 patients with children asthma and 50 controls were tested for exon Ⅱ,Ⅲ of α1-antichymotrypsin by PCR,RFLP to analyze AACT encoding gene.Results：Bochum-1,Bonn-1 mutations of the AACT gene were not found in any of the subjects.Conclusion：There is no relationship between α1-antichymotrypsin gene mutations including Bochum-1,Bonn-1 and 50 han asthmatic children in Kunming. |
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| Keywords: | Children asthma α1-antichymotrypsin Gene mutation PCR |
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