结缔组织生长因子在糖尿病大鼠肾脏中的表达及其意义

目的 :研究结缔组织生长因子 (CTGF)在链脲佐菌素 (STZ)诱导的糖尿病大鼠肾脏中的表达及其与肾小球肥大的关系。方法 :将SD大鼠随机分为正常对照 (N)组与糖尿病肾病(DN)组。大鼠单侧肾切除后 ,腹腔注射STZ诱导糖尿病模型 ,观察第 4、8、12周血糖、体重、尿白蛋白排泄 (Ualb)和 2 4h尿蛋白定量 (2 4hUpro)的变化 ,同时观察 12周时肌酐清除率(Ccr)、肾小球面积及体积、肾重、肾组织总蛋白含量和肾皮质CTGF表达的改变。结果 :DN组 2 4hUpro、Ualb和Ccr较N组明显增加 (P <0 .0 1) ,肾小球面积及体积、肾重和肾组织总蛋白含量均较N组显著增加 (P <0 .0 5或P <0 .0 1)。免疫组织化学半定量检测结果显示 ,DN组肾皮质CTGF表达较N组明显增加 (P <0 .0 1) ,CTGF表达与肾小球体积及 2 4hUalb呈显著正相关关系 (均为P <0 .0 5 )。结论 :糖尿病大鼠早期肾脏CTGF表达增加可能是DN发生的重要机制

Expression of connective tissue growth factor in the kidney of diabetic rats

Objective To investigate the expression of connective tissue growth factor (CTGF) in the kidney of STZ induced diabetic rats and its relationship with the early renal hypertrophy.Methods Sprague Dawley (SD) rats were randomly divided into two groups,normal control (group N),diabetic nephropathy (group DN).Diabetes was induced by injection of STZ intraperitoneally after rats had received uninephrectomy.Blood glucose,body weight, urinary albumin excretion (Ualb),24 hour proteinuria (24?h?Upro) were observed respectively in the rats in 4,8,12 weeks of the experiment.Creatinine clearance (Ccr), the kidney weight,glomerular area and volume,renal tissue protein contents and renal expression of CTGF were determined in 12 weeks,when the rats were sacrificed.Results Ualb excretion,24?h?Upro and Ccr in DN group were significantly increased compared with that in N group ( P <0.01 respectively).Kidney weight, glomerular area and volume,renal tissue protein contents were markedly increased in DN group compared with that in N group ( P <0.05, P <0.01 respectively).Semi quantitative assessment of the immuno histochemical staining showed that glomerular expression of CTGF in group DN was significantly increased compared to that in group N( P <0.01),Furthermore,the expression of CTGF is positively correlated with the increasing of glomerular volume and proteinuria ( P <0.01 respectively).Conclusion Expression of CTGF is increased in early diabetic rats,which is possibly involved in the development of diabetic nephropathy.