低分化胃癌患者血清中相关标志物的筛查和鉴定

目的 初步探讨蛋白质组技术在血清研究中的应用，建立低分化胃癌患者血清和健康者血清双向凝胶电泳图谱；筛查并鉴定低分化胃癌患者血清和健康者血清中的差异表达蛋白质，期望从中发现有意义的胃癌分子标志物。方法 采用双向凝胶电泳（2-DE）分离低分化胃癌患者血清和健康者血清总蛋白，银染显色，PDQuest软件分析，从中选取差异表达蛋白质，通过基质辅助激光解析电离飞行时间质谱（MALDI-TOF-MS）鉴定差异表达的蛋白质。结果 获得重复性和分辨率较好的血清双向凝胶电泳图谱；鉴定得到6个仅在胃癌组血清中表达的蛋白质和4个仅在对照组血清中表达的蛋白质。结论 在低分化胃癌患者血清与健康者血清中存在差异表达蛋白质，利用蛋白质组学这一高通量的筛查技术可望从中发现与低分化胃癌相关的标志物。

Identification of biomarkers in the serum of the patients with poorly differentiated gastric cancer

Objective To investigate the application of proteomics in the field of serology,and to screen the differential expression proteins related with poorly differentiated gastric carcinoma. Methods Two- dimensional electrophoresis (2- DE) was applied to segregate the total proteins in the serum form gastric cancer patients and health volunteers. After staining,the differential expression proteins were analyzed using PDQuest software,and identified using matrix- assisted laser desorption/ionization- time of flight mass spectrometry (MALDI- TOF- MS). Results Electrophoresis figures with high resolution and reproducibility were obtained. Six differential expression proteins were found only in the serum from gastric cancer patients,while four other proteins from healthy volunteers. Conclusions Protein expression is differential in the serum from the gastric cancer patients and health volunteers. It is hopeful to find the biomarkers related with poorly differentiated gastric carcinoma using proteomics.