| 灯盏花素增强马利兰抑制HL-60细胞作用的实验研究 |
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| 引用本文： | 任亮,马菲,李晓莉,张延新,杜钢军. 灯盏花素增强马利兰抑制HL-60细胞作用的实验研究[J]. 中国药房, 2010, 0(19): 1749-1751 |
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| 作者姓名： | 任亮 马菲 李晓莉 张延新 杜钢军 |
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| 作者单位： | 漯河医学高等专科学校;河南漯河市源汇区妇幼保健院;河南大学药学院; |
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| 摘 要： | 目的:研究灯盏花素增强马利兰抑制HL-60细胞的作用。方法:MTT法检测细胞活性,流式细胞仪检测细胞凋亡情况,Western blotting法检测肿瘤抑制基因(p53)/B细胞淋巴瘤-白血病-2(Bcl-2)的表达,ELISA、比色法和分光光度法分别检测细胞色素C、Caspase-3、Caspase-8和谷胱甘肽(GSH)的含量。结果:灯盏花素能增强马利兰抑制HL-60细胞生长的作用,促进小鼠淋巴细胞对马利兰的抵抗,上调p53/Bcl-2表达比率,激活Caspase-3和Caspase-8,升高细胞色素C,降低GSH。结论:灯盏花素对马利兰抗肿瘤具有增效减毒作用,其机制可能与激活肿瘤耐药细胞凋亡通路有关。
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| 关 键 词： | 灯盏花素 马利兰 HL-60细胞 淋巴细胞 |
Study on Promotion Effect of Breviscapine on Myleran Inhibiting the Growth of HL-60 Cell |
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| REN Liang,LI Xiao-li,ZHANG Yan-xin MA Fei DU Gang-jun. Study on Promotion Effect of Breviscapine on Myleran Inhibiting the Growth of HL-60 Cell[J]. China Pharmacy, 2010, 0(19): 1749-1751 |
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| Authors: | REN Liang LI Xiao-li ZHANG Yan-xin MA Fei DU Gang-jun |
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| Affiliation: | REN Liang,LI Xiao-li,ZHANG Yan-xin(Luohe Medical College,Luohe 462002,China) MA Fei(Luohe Yuanhui District Maternal , Child Health Hospital of Henan Province,China) DU Gang-jun(Pharmaceutical College of Henan University,Kaifeng 475001,China) |
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| Abstract: | OBJECTIVE:To observe promotion effect of breviscapine on myleran inhibiting the growth of HL-60 cell.METHODS:The activity of HL-60 cells was tested by MTT assay and cell apoptosis was determined by flow cytometry.The expression of p53/Bcl-2 was assessed by Western blotting.The level of cytochrome C,Caspase-3,Caspase-8 and GSH were measured by ELISA,colorimetry assay and spectrophotometry respectively.RESULTS:Breviscapine could promote inhibition effect of myleran on the growth of HL-60 cell and enhance drug... |
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| Keywords: | Breviscapine Myleran HL-60 cell Lymphocyte |
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