漏声表面波生物传感器不同检测方法的实验研究

目的构建双通道漏声表面波生物传感器液相检测技术平台。方法以人乳头状瘤病毒为检测对象,传感器检测系统运用相位、频率及延迟线3种检测方法对其进行实时检测;利用扫描隧道显微镜观察传感器裸金表面、固定巯基化探针以及核酸杂交后的金膜表面结构的微观变化。结果相位和频率两种检测方法较好;扫描隧道显微镜下可以看到裸金膜表面金原子排列有序;探针分子分布密度增加;杂交后寡核酸分子密度更为密集。结论成功构建了漏声表面波生物传感器液相检测技术平台并运用多种检测方法;扫描隧道显微镜可以形象直观地对传感器金膜表面、核酸杂交进行直接观察,结果进一步证实了实验结论的正确性。

Leaky Surface Acoustic Wave Biosensor and Its Different Detection Methods

OBJECTIVE To design the detection system of the leaky surface acoustic wave (LSAW) biosensor in liquid phase. METHODS Real time monitoring of HPV reaction was performed by using the LSAW biosensor system and the three detecting methods including phase, frequency and delayed time; probe was immobilized on the gold electrode surface of LSAW biosensor by thiol method, and hybridized with corresponding target sequence. Then scanning tunneling microscope(STM) was used to observe microcosmic change on the surface of LSAW biosensor during the process of probe immobilization and hybridization, and also the naked gold membrane. RESULTS Detection with phase and frequency was two good choices; STM could directly observe the evenly arrayed gold particles on the naked gold surface. After the probe was immobilized, the density of the probe was improved. When the probe hybridized with target sequence, the density of the nucleic acid was higher, the length of the molecule was significantly increased. CONCLUSIONS The detection system of the LSAW biosensor in liquid phase is successfully constructed and uses three detecting methods; STM can directly observe the gold membrane of LSAW sensor, the process of DNA probe immobilization, and hybridization with target sequence. And the results further approve the correctness of our experimental conclusions.