GDNF诱导分化骨髓基质细胞对PD大鼠的治疗作用

目的探讨胶质细胞源性神经营养因子（GDNF）诱导分化骨髓基质细胞（BMSCs）脑内移植对帕金森病（PD）模型的治疗作用。方法体外培养、分离和纯化的BMSCs，用5-溴脱氧尿核苷（BrdU）标记和GDNF诱导分化。分别将经GDNF诱导分化（A组）和未经GDNF诱导分化（B组）的BMSCs移植到PD大鼠模型纹状体区。另设生理盐水对照组（C组）和PD模型对照组（D组）。于不同时间点检测大鼠旋转行为变化，运用免疫荧光组织化学方法分析比较各组纹状体内酪氨酸羟化酶（TH）阳性细胞数量。结果①旋转行为检测显示，C组和D组在所有时间点未见明显变化；在移植后7～30d，A组和B组分别与C组和D组比较，旋转行为明显减少（P〈0．05）；A组比B组旋转行为减少更为显著（P〈0．05）。②免疫荧光组织化学检测显示，A组与B组比较，GFAP和TH阳性细胞数量明显增多（P〈0．05）。但各组自身各时程比较数量变化无统计学意义（P〉0．05）。结论A组的BMSCs脑内移植有效地改善了PD大鼠模型的旋转行为，提高了移植后TH阳性细胞的数量。

Therapeutic effects of GDNF-induced and differentiated bone marrow stromal cell transplantation on rat models of Parkinson''s disease

Objective To explore therapeutic effects of glial cell line-derived neurotrophic factor(GDNF) induced and differentiated bone marrow stromal cell(BMSCs) transplantation on rat models of Parkinson's disease(PD).Methods The PD rats were divided randomly into four groups: GDNF-induced and differentiated BMSCs graft group(group A),without GDNF-induced and differentiated BMSCs graft group(group B) and physiological saline control group(group C) and PD model control group(group D).The rotation test and immunofluorescence staining were performed.Results Frequency of rotation was significantly less in group A or group B than that in control group(P<0.05).5-bromodexyuridine(Brdu)-BMSCs and tyrosine hydroxylase(TH) positive cells appeared.The number of TH-positive cells in group B was significantly fewer than that of group A(P<0.05).Such cells were not found in the control group.Conclusion The rotational behavior can be effectively ameliorated and the number of TH positive cells is elevated by GDNF-induced and differentiated BMSCs graft.