| 改良PAGE法用于人类珠蛋白多态性研究 |
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| 引用本文： | 易志勤,南国华,陈松森. 改良PAGE法用于人类珠蛋白多态性研究[J]. 江西医学院学报, 2003, 43(2): 24-25,29 |
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| 作者姓名： | 易志勤 南国华 陈松森 |
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| 作者单位： | [1]江西医学院门诊部，江西南昌330006 [2]江西医学院生化教研室，江西南昌330006 [3]中国医学科学院基础医学研究所血红蛋白研究室，北京100005 |
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| 摘 要： | 目的：探讨人类新生儿HbF中珠蛋白链简便、快速、准确的分离方法。方法：对传统的含Triton X—100的酸性聚丙烯酰胺凝胶电泳法的凝胶系统作了进一步的改进，其中Triton X—100和TEMED等主要物质的浓度作了调整，不同于其它文献报导的。结果：该改良法能使凝胶聚合时间缩短、一次电泳时间从6h缩短为2h，HbF中各珠蛋白链得到良好分离，电泳条带清晰，无拖尾现象；结论：该方法操作简便，分离效果好，电泳时间缩短，极大地提高了研究效率。
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| 关 键 词： | 改良PAGE法 人类 珠蛋白多态性 研究 聚丙烯酰胶凝胶电泳 |
| 文章编号： | 1000-2294(2003)02-0024-02 |
A Modified PAGE Method Used in the Human Globin Heterogeneity Researching |
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| Yi Zhi qin,NAN Guo hua ,CHEN Song sen. A Modified PAGE Method Used in the Human Globin Heterogeneity Researching[J]. Acta Academiae Medicinae Jiangxi, 2003, 43(2): 24-25,29 |
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| Authors: | Yi Zhi qin NAN Guo hua CHEN Song sen |
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| Affiliation: | Yi Zhi qin,NAN Guo hua 2,CHEN Song sen 3 |
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| Abstract: | Objective:To seek a method for separating human globin chains in HbF in the new born babies.Methods:Concentration of the main matters(Triton X 100 and TEMED)in the polyacrylamide system in traditional polyacrylamide gel electrophoresis(PAGE)was modified.Results: The globin chains can be well separated by this modified method and the electrophoresis time has been shorted down (from 6 hours to 2 hours ).Conclusion:This method is not only seperating the globin chains well, but also easy to operate and shortens the time of electrophoresis,So it greatly increases the research efficiency in this field. |
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| Keywords: | PAGE method globin heterogeneity |
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