新生儿红细胞免疫功能的测定

目的　探讨红细胞免疫功能在新生儿期的变化及相关因素。方法　随机选择 2 0名足月正常产的新生儿为新生儿组。采用肝素抗凝脐带血检测红细胞C3b补体受体黏附率 (RC3bR)和红细胞免疫复合物黏附率 (RICR) ,使用普利生LBY—N6B全自动血流变仪 ,用肝素抗凝脐带血检测血液流变学。同时选择 2 0名健康儿童为正常对照组。所获资料用 x±s表示 ,并行组间均数比较 ,新生儿组RC3bR与血液流变学各项指标行直线相关统计学处理。结果　新生儿组RC3bR、RICR与对照组比较 :RC3bRt =0 79,RICRt =1 73,P >0 0 5 ,差异无统计学意义。新生儿组血液流变学与对照组比较 :全血黏度t =2 37,P <0 0 5 ;红细胞聚集指数t =3 0 0 ,红细胞变形指数t=2 85 ,红细胞压积t=8 76 ,血沉t=15 0 1,均P <0 0 1,差异有统计学意义。新生儿组RC3bR与血液流变学各项指标直线相关统计学处理结果 :全血黏度r =- 0 77,血浆黏度r =- 0 75 ,P <0 0 1;红细胞变形指数r=0 5 3,P <0 0 5 ,差异有统计学意义。提示RC3bR与全血黏度、血浆黏度呈负相关 ,与红细胞变形能力呈正相关。结论　新生儿出生时 ,红细胞免疫功能已发育成熟 ,任何原因导致的血黏度增高、红细胞变形能力下降 ,均可使红细胞免疫功能降低。

Determination of erythrocytic immune function in newborn

Objective Studying the changes of erythrocytic immune adherent function in normal newborn.Methods Twenty newborns of normal term birth were selected at random,Their heparin-anticoagulant cord blood samples were collected,the adherent rate of complement-Ⅲ receptor on the surface of RBC (RC 3b R) and the immune complex adherent rate of RBC (RICR) were examined with Guo′s method;hemorrheological data were examined with Pulisheng automatic machine,20healthychildrenagedfrom3to14yearswerechosenasthecontrolgroup.Results Their RC 3b R and RICR showed nodifferencebetweenthenewbornsandthecontrolgroup (RC 3b R t=0.79,P>0.05,RICR t=1.73,P>0.05),but the difference in hemorrheologic data showed a great significance between the two groups whole blood viscosity t=2.37,RGI t=3.00,RCI t=2.85,HCT t=8.76,ESR t=15.01,all P<0.01).Inthenewborngroup,therewasapositivecorrelationbetweenRC 3b RandRBCdeformability (r=0.53,P<0.05) ,negativecorrelationbetweenRC 3b Randwholebloodviscosityorplasmaviscosity(wholebloodviscosity r=-0.77,r=-0.75,all P<0.05).Conclusion Erythrocytic immune function has matured in a newborn infant,but an increased blood viscosity and/or a decreased RBC deformability that are induced by any causes can suppress the erythrocytic immune function.