光动力疗法对人卵巢癌细胞系Skov3凋亡的影响

目的 通过对经光动力疗法(PDT)处理后卵巢癌细胞系Skov3发生凋亡情况的研究,探讨光动力学疗法对体外培养的人卵巢癌细胞的影响. 方法 采用不同能最激光对不同浓度光敏剂(5-ALA)处理过的卵巢癌细胞系(Skov3)进行照射,48 h后应用光镜、电镜进行形态学观察,同时利用流式细胞仪检测细胞凋亡情况. 结果 倒置显微镜下经光动力学处理后Skov3细胞体积缩小,细胞变形、皱缩,细胞核浓缩、深染,染色质密集成斑块状,细胞间失去彼此连接,透射电镜下可见致密的染色质沿核膜下聚集,有凋亡小体形成.流式细胞仪定量分析最大细胞凋亡率为60.5%. 结论 PDT通过诱导捅亡对体外培养的Skov3细胞产生杀伤作用.

Apoptosis in ovarian cancer cell lines after photodynamic therapy in vitro

Objective The apoptosis of ovarian cancer cell line Skov3 under the photodynamic therapy were studied to explore the effect of photodynamic therapy in gynecological cancer. Methods Ovarian cancer cell line Skov3 treated with different doses of photosensitizer 5- ALA were radiated by laser with difference doses, then morphological characteristics were studies by light microscopy and electronic microscopy, and cell apoptosis were studied with flow cytometry (FCM). Results The cytomorphosis and shrinkage, nucl...