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| 间充质干细胞成血管因子受体表达特征及其意义 | |
| 引用本文： | 刘春风,陈亮华,杨伟,唐俊明,杨建业,王家宁,孔霞,郭凌郧,郑飞,黄永章.间充质干细胞成血管因子受体表达特征及其意义[J].陕西医学杂志,2010,39(9):1107-1110. |
| 作者姓名： | 刘春风 陈亮华 杨伟 唐俊明 杨建业 王家宁 孔霞 郭凌郧 郑飞 黄永章 |
| 作者单位： | 1. 郧阳医学院附属人民医院临床医学研究所,十堰,442000 2. 郧阳医学院生理学教研室;郧阳医学院第一临床学院 3. 郧阳医学院生理学教研室 |
| 基金项目： | 国家自然科学基金，郧阳医学院大学生科研项目 |
| 摘 要： | 目的:探讨间充质干细胞成血管因子受体表达特征及其意义。方法:采用经典的全骨髓贴壁法培养骨髓源间充质干细胞(MSC),通过成骨、成脂肪等多向诱导分化以及流式细胞分析其表面标记(CD34、CD90、CD29)等鉴定MSC特征;以P1-P17MSC为实验材料,利用RT-PCR的方法检测PDGFR、VEGFR和NRP1的表达。利用Transwell体外迁移体系初步探索VEGF在MSC迁移中的作用,同时利用MTT法评价VEGF对MSC增值的作用。结果:培养的MSC呈现出CD90、CD105强阳性,具有成骨、成脂肪等多向分化能力;强表达PDGFR-β和NRP1、不表达VEGFR(Flk1和Flt1)和PDGFR-α,VEGF促进了MSC的增殖和迁移。结论:VEGF可能通过PDGFR-NRP1促进了MSC的增值和迁移。 |
| 关 键 词： | 干细胞/生理学 中胚层 血管生成因子/代谢 细胞培养 动物 实验 大鼠 |
The characteristical of angiogenic factor receptor expression in mesenchymal stem cell |
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| Institute of Clinical Medicine,the Affiliated People''s Hospital,Yunyang Medical College Liu Chunfeng Chen Lianghua Yang Wei et al.The characteristical of angiogenic factor receptor expression in mesenchymal stem cell[J].Shaanxi Medical Journal,2010,39(9):1107-1110. | |
| Authors: | Institute of Clinical Medicine the Affiliated People's Hospital Yunyang Medical College Liu Chunfeng Chen Lianghua Yang Wei |
| Institution: | Institute of Clinical Medicine,the Affiliated People's Hospital,Yunyang Medical College(Shiyan 442000) Liu Chunfeng Chen Lianghua Yang Wei et al |
| Abstract: | Objective:To explore the characteristical of angiogenic factor receptor expression in mesenchymal stem cell(MSC).Methods:MSC culture was performed with the classical adhering method;characteristic of MSC was identified through multi-differentiation and surface marker assay(CD90,CD133,CD34,CD29);the expression of PDGFR,VEGFR and NRP1 in MSC were analyzed by RT-PCR,the proliferation rate of MSC was measured after VEGF treatment by MTT assay,and the VEGF dose-dependent of MSCs migration were observed by using ... |
| Keywords: | Stem cells/physiology Mesoderm Angiogenesis factor/metabolism Cell culture Animals laboratory Rats |
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