栀子厚朴汤及相关药对和单味药材的指纹图谱研究及多指标定量分析

目的：建立栀子厚朴汤及相关药对和单味药材的指纹图谱研究和多指标定量分析方法。方法：采用Lichrospher C18柱（250 mm×4.6 mm,5μm）,柱温为25℃;流动相为甲醇-0.1%乙酸水溶液,流速为1.0 mL.min-1,梯度洗脱;检测波长为238 nm。结果：在上述分析条件下,各样品中共存成分实现了基线分离;栀子苷、橙皮苷、和厚朴酚以及厚朴酚质量浓度分别在9~1 500、15.6~650、4.5~750和6~1 000 mg.L-1的范围内与色谱峰面积呈线性关系,检测限分别为1.6、3.1、0.9和0.6 mg.L-1。多指标定量分析结果显示,全方及药对和单味药材中相应指标性成分的含量存在差异。结论：所建立的方法重复性好,灵敏度、精密度和准确度均较高,可用于栀子厚朴汤及药对和单味药材的指纹图谱研究及多指标定量分析。

Studies on Fingerprints and Multi-index Quantitative Analysis of Zhi-Zi-Hou-Pu Decoction as Well as the Related Drug Pairs and Single Medicine

Objective： To establish a RP-HPLC method for the studies on fingerprints and multi-index quantitative analysis of Zhi-Zi-Hou-Pu decoction （ZZHPD） as well as the related drug pairs and single medicine. Methods： The Lichrospher Cls column （250 mm × 4.6 mm, 5 p,m） was used, and the column temperature was kept constant at 25 ℃. The mobile phase consisted of a mixture of methanol and 0. 1% aqueous acetic acid with a gradient program at a flow rate of 1.0 mL-min-1. The UV detection wavelength was set at 238 nm. Results： Coexisting compounds were baseline-separated under the above chromatographic conditions. The calibration curves for geniposide, hesperidin, honokiol, and magnolol were linear over the range of 9-1 500, 15.6-650, 4. 5-750, and 6-1 000 mg·L^-1, respectively. The limit of detection for them was 1.6, 3.1,0.9, and 0. 6 mg·L^-1, respectively. The content of the four components was different among different combinations of ZZHPD. Conclusion： The method is reproducible, sensitive, precise and accurate. It can be applied to the study on fingerprints and multi-index quantitative analysis of ZZHPD as well as the related drug pairs and single medicine.