| 乳汁HBV-DNA定量时标本处理的优选及临床应用 |
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| 引用本文： | 方有兵,黄晨艳,刘贵育.乳汁HBV-DNA定量时标本处理的优选及临床应用[J].中国妇幼保健,2011,26(17):2664-2666. |
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| 作者姓名： | 方有兵 黄晨艳 刘贵育 |
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| 作者单位： | 1. 安徽医科大学第一附属医院检验科,安徽,合肥,230022
2. 合肥市妇幼保健院医务科 |
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| 基金项目： | 安徽省合肥市科技局医学应用性研究项目 |
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| 摘 要： | 目的:选择灵敏度高、重复性较好的乳汁HBV-DNA定量标本提取方法应用于乙肝携带产妇的乳汁检测。方法:选择14例乙肝"大三阳"产妇乳汁,每份分为乳汁原标本,乳汁离心后中层,乳汁离心后下层沉淀混合液3组,分别提取HBV-DNA并检测含量,用秩和检验分析结果的差异;用乳汁离心后中层对316例乙肝产妇检测HBV-DNA含量。结果:乳汁离心后中层清液灵敏度高于乳汁原样本,乳汁离心后中层清液重复性高于下层沉淀混合液。在316例乙肝产妇中,血清HBsAg+、HBeAg+、HBcAb+组,其乳汁HBV-DNA阳性率为77.60%;血清HBsAg+,HBeAb+,HBcAb+组其乳汁HBV-DNA阳性率为0.73%,血清HBsAg+,HBeAg+组乳汁HBV-DNA阳性率为80.00%,血清HBsAg+和HBcAb+组乳汁HBV-DNA阳性率为5.17%,其余组别乳汁HBV-DNA阳性率皆为0。结论:乙肝携带者产妇的乳汁可用于评估母乳喂养安全性。在乙肝产妇乳汁中,以其血清HBsAg+、HBeAg+、HBcAb+者和血清HBsAg+、HBeAg+者母乳喂养小儿被传染乙肝病毒的风险较大。
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| 关 键 词： | 乳汁 HBV-DNA检测 离心沉淀 乙肝产妇 母乳喂养 |
Optimization and clinical application of treatment of specimens in HBV-DNA quantitation |
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| FANG You-Bing,HUANG Chen-Yan,LIU Gui-Yu.Optimization and clinical application of treatment of specimens in HBV-DNA quantitation[J].Maternal and Child Health Care of China,2011,26(17):2664-2666. |
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| Authors: | FANG You-Bing HUANG Chen-Yan LIU Gui-Yu |
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| Institution: | FANG You-Bing,HUANG Chen-Yan,LIU Gui-Yu.Clinical Laboratory,the First Hospital Affiliated to Anhui Medical University,Hefei 230022,Anhui,China |
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| Abstract: | Objective:To choose a method with high sensitivity and good repeatability of drawing HBV-DNA quantitative specimens from colostrum used for colostrum detection of lying-in women with hepatitis B.Methods:14 colostrum specimens from 14 lying-in women carrying positive HBsAg,HBeAg and HBcAb were selected,and every colostrum specimen was divided into 3 groups: original specimen,centrifugal specimen at middle level and centrifugal sediment at low level,then HBV-DNA was extracted respectively and the content was ... |
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| Keywords: | HBV-DNA |
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