丙泊酚麻醉对肝硬化大鼠苏醒时间的影响

目的通过制备大鼠肝硬化模型,观察肝硬化大鼠对丙泊酚麻醉后苏醒时间的影响。方法健康雄性SD大鼠60只,体重180-220 g,鼠龄10-12周,随机分为正常对照组（C组,n=10只）和肝硬化组（M组,n=50只）。M组采用四因素法制备大鼠肝硬化模型,模型制备成功后尾静脉注射丙泊酚12.368 mg/kg（2ED50）,记录两组大鼠的麻醉作用时间,分别以翻正反射消失和翻正反射恢复作为判断丙泊酚麻醉起效和麻醉苏醒的标准。下腔静脉取血3 ml,全自动生化分析仪检测血清前白蛋白（PA）的浓度。结果与C组比较,M组大鼠翻正反射恢复时间明显延长（P〈0.05）,与C组比较,M组大鼠的PA浓度明显降低（P〈0.05）。结论肝硬化大鼠可使丙泊酚麻醉后的苏醒时间延长。

Effect of hepatic cirrhosis on awaking-time in rats by propofol anesthesia

Objective To establish a model of hepatic cirrhosis in rats,and to observe the effect of hepatic cirrhosis on awaking-time in rats by propofol anesthesia.Methods Sixty healthy male SD rats weighting 180-220 g,aged 10-12 weeks were randomly divided into control group（group C,n=10） and hepatic cirrhosis group（group M,n=50）.The model of hepatic cirrhosis was established by using four factors.Propofol was injected intravenously with 12.368 mg/kg（2ED50） after the model established successfully.The loss of forepaw righting reflex and recovery of forepaw righting reflex were respectively defined as onset and recovery of anesthesia in rats.The rats were anesthetized with propofol and then blood sample was taken from inferior vena cava for prealbumin test.Results The awaking time was significantly longer in group M than that in group C.The prealbumin was significantly lower in group M than that in group C.Conclusion The awaking-time is prolonged in hepatic cirrhosis rats by propofol anesthesia.