| 高效液相色谱法同时测定白花蛇舌草中两组分的含量 |
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| 引用本文： | 罗新根,蒋琳兰,赵树进. 高效液相色谱法同时测定白花蛇舌草中两组分的含量[J]. 中国药房, 2004, 15(3): 175-176 |
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| 作者姓名： | 罗新根 蒋琳兰 赵树进 |
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| 作者单位： | 广州军区广州总医院药学部,广州市,510010 |
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| 摘 要： | 目的 :建立白花蛇舌草中齐墩果酸与熊果酸的含量测定方法。方法 :色谱柱为NucledureC18 柱 ,流动相为乙腈 -磷酸盐缓冲液 (45∶55) ,检测波长为220nm。结果 :齐墩果酸的平均回收率为 (96 79±2 42) % ,熊果酸的平均回收率为 (97 15±2 33) %。结论 :本方法可同时测定齐墩果酸和熊果酸的含量 ,可用于药材质量控制
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| 关 键 词： | 白花蛇舌草 齐墩果酸 熊果酸 含量测定 高效液相色谱法 |
| 文章编号： | 1001-0408(2004)03-0175-02 |
| 修稿时间： | 2003-03-29 |
Simultaneous Determination of Oleanolic Acid and Ursolic Acid in Hedyotis Diffusa with HPLC |
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| LUO Xingen,JIANG Linlan,ZHAO Shujin. Simultaneous Determination of Oleanolic Acid and Ursolic Acid in Hedyotis Diffusa with HPLC[J]. China Pharmacy, 2004, 15(3): 175-176 |
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| Authors: | LUO Xingen JIANG Linlan ZHAO Shujin |
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| Abstract: | OBJECTIVE:To develop a RP-HPLC method for simultaneous determination of oleanolic acid and ursolic acid in Hedyotis diffusa.METHODS:The optimum HPLC conditions were as follows:Nucledure C 18 column(4.6mm×150mm);mobile phase:acetonitrile-potassium phosphate,pH=7.84(45∶55);detection wavelength:220nm.RESULTS:The average recoveries of oleanolic acid and ursolic acid were(96.79±2.42)%and(97.15±2.33)%respectively.CONCLUSION:The met_ hod could detect oleanolic acid and ursolic acid in Hedyotis diffusa simultaneously.It could be used in the quality control of the herb. |
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| Keywords: | Hedyotis diffusa oleanolic acid ursolic acid determination of content HPLC |
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