佛手茎尖微嫁接技术研究

目的探讨佛手茎尖微嫁接技术,为佛手无病苗木的培育奠定基础。方法黑暗条件下培养柠檬实生苗用作砧木,以佛手茎尖为接穗,采用倒"T"字形法进行茎尖微嫁接试验,并对影响嫁接成活率的因素进行研究。结果成功获得了佛手茎尖微嫁接苗,并优化了微嫁接的条件:选取苗龄为14d的柠檬实生苗为砧木,嫁接成活率较高;以带4个叶原基的佛手茎尖为接穗,效果较好,嫁接成活率可达65.2%;茎尖微嫁接后,切口外缠parafilm膜,能明显提高成活率。结论建立了佛手茎尖微嫁接的方法,获得了嫁接成活苗。

Technique for shoot-tip grafting of Citrus medica var.sarcodactylis

Objective To study the technique for shoot-tip grafting of Citrus medica var.sarcodactylis in order to lay a foundation for culturing disease-free plantlets.Methods Using dark grown lemon seedling as rootstock and shoot-tip of C.medica var.sarcodactylis as scion,the shoot-tip grafting was executed by grafting a scion in an inverted-T incision of lemon seedling.And the factors affecting the survival rate of shoot-tip grafting were studied.Results The micro-grafted plantlets of C.medica var.sarcodactylis were achieved.It was suggested that the more survival rate of grafting was obtained by using 14-day-old dark grown seedlings as rootstocks.The scions consisted of the apical meristem plus four leaf primordia were optimal for grafting,with survival rate of 65.2%.Moreover,the survival rate of grafting could be increased obviously if the grafting-incisions were twisted with parafilm.Conclusion A procedure of shoot-tip grafting in vitro has been established in C.medica var.sarcodactylis and micro-grafted survival plantlets have been obtained.