Effects of fluoride on membrane permeability and brush border enzymes of rat intestine in situ |
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| Authors: | R M Shayiq H Raza A M Kidwai |
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| Affiliation: | 1. Center for Endemic Disease Control, Chinese Center for Disease Control and Prevention, Harbin Medical University, Harbin 150081, Heilongjiang Province, China;2. Key Lab of Etiology and Epidemiology, Education Bureau of Heilongjiang Province & Ministry of Health (23618504), Harbin Medical University, Harbin 150081, Heilongjiang Province, China;3. Institution of Environmentally Related Diseases, Harbin Medical University, Harbin, Heilongjiang Province, China;4. Heilongjiang Provincial Key Laboratory of Trace Elements and Human Health, Harbin Medical University, Harbin 150081, Heilongjiang Province, China;1. Departamento de Bioquímica e Microbiologia, Instituto de Biociências, UNESP – São Paulo State University, Avenida 24 A, 1515–Bela Vista, 13506-900, Rio Claro-SP, Brazil;2. Faculdade de Ciências Agrárias e Tecnológicas, UNESP – São Paulo State University, Rodovia Comandante João Ribeiro de Barros (SP 294), Km 651, 17900-000, Dracena-SP, Brazil;1. Department of Regenerative Medical Science, School of Pharmaceutical Sciences, Jilin University, Changchun, 130021, People’s Republic of China;2. First Clinical Hospital, Jilin University, Changchun, 130021, People’s Republic of China |
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| Abstract: | The effect of different concentrations of sodium fluoride (12, 24, 48 and 96 mM), instilled into the ligated intestine of anaesthetized rats for 30 min, on intestinal permeability and some brush border enzymes was investigated. A concentration-dependent change in permeability was observed; there was an increase in the volume of luminal fluid and altered net transport of Na+ and K K+ ions. The change in permeability was accompanied by increased protein, sialic acid and nucleic acid accumulation in the luminal fluid. A striking loss of brush border alkaline phosphatase (41%) sucrase (59%) and gamma-glutamyl transpeptidase (73%) activities was observed at 96 mM fluoride with a corresponding increase in the activity of these enzymes in luminal fluid, while 12 mM fluoride did not produce any significant effect. This loss was probably not due to an inhibition of the enzymes by fluoride since in vitro experiments did not produce any such effect over a range of NaF concentrations (0-32 mM) except on alkaline phosphatase activity at the 32 mM NaF concentration. The studies, therefore, suggest that the loss of brush-border enzyme activities observed in situ was most probably due to membrane damage caused by the high fluoride concentration. |
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