前S1抗原与乙型肝炎病毒DNA及e抗原的关系

目的探讨乙型肝炎病毒（HBV）前s1抗原（Pre-S1Ag）与HBV-DNA、乙型肝炎e抗原（HBeAg）的关系，了解检测Pre-S1Ag的临床意义。方法用酶联免疫吸附试验检测Pre-S1Ag、HBeAg，荧光定量聚合酶链反应检测HBV-DNA。结果（1）在205例HBV-DNA阳性人群中，Pre-SlAg总阳性率为84．3％，HBeAg总阳性率为79．0％，两者比较差异无统计学意义（P〉0．05）。（2）当HBV-DNA〉5×10^4copy／mL时，Pre-S1Ag阳性率为88．3％，HBeAg阳性率为96．6％，两者比较差异无统计学意义（P〉0．05）；当HBV-DNA＜5×10^4copy／mL时，Pre-S1Ag阳性率为78．8％，HBeAg阳性率为54．1％，两者比较差异有统计学意义（P〈0．05）。（3）在HBV-DNA水平不同的两组中，HBeAg的阳性率分别为96．6％和54．1％，差异有统计学意义（P〈0．01）；Pre-S1Ag阳性率分别为88．3％和78．8％，差异无统计学意义（P〉O．05）。结论前S1抗原与HBV-DNA有高度的相关性，可以作为HBV存在和复制的指标；前S1抗原与HBeAg联合检测对判断HBV的存在和复制更有价值。

Relationship between the Pre-S1 antigen and HBV-DNA as well as HBeAg

Objective To investigate the relationship between the Pre-S1 antigen （Ag） and HBV-DNA as well as HBeAg, and to understand the clinical significance of Pre-S1 antigen. Methods ELISA was used to measure Pre- SlAg and HBeAg, while HBV-DNA was determined by applying quantitative PCR assay. Results The total positive rate of Pre-S1 antigen and HBeAg was 84.3% and 79.0% respectively in 205 patients with HBV-DNA positive. The positive rate of Pre-S1 antigen and HBeAg was 88.3% and 96.6% respectively in HBV-DNA〉5 X 10^4 copy/mL group was 88.3%, and 78.8% and 54.1% respectively in HBV-DNA〈5 X 10^4 copy/mL group. The difference of HBeAg was statistically significant between the above two groups （P〈0. 01）, whereas there wasn＇t statistical difference of Pre-S1 antigen between the two groups （P〉0.05）. Conclusion Pre-S1 Ag and HBV-DNA has high relevance. Pre-S1 Ag may be used as a marker of HBV existence and duplication. Combined detection of Pre-S1 Ag and HBV-DNA is of important value in identifying the existence and duplication of HBV.