葡萄糖调节蛋白94在人胚胎发育中的表达

目的 为探讨葡萄糖调节蛋白９４（ＧＲＰ９４）在胚胎发育中的作用，研究了１０－３１周人胎组织ＧＲＰ９４的定位及表达。方法 用ＳＰ免疫组织化学方法。结果 结肠粘膜上皮，肾小管上皮及肝细胞的胞质内，在１０周已有ＧＲＰ９４的表达，１８－２０周时表达增多，一直维持较高水平。胃粘膜上皮及心肌细胞也有较强的表达。

STUDY ON THE EXPRESSION OF GLUCOSE-REGULATED PROTEIN94 IN DEVELOPMENT OF THE HUMAN FETUSES

Objective Glucose regulated protein94(GRP94),which belongs to HSP90 family, is resided to the endoplasmic reticulum(ER) as molecular chaperones. We examined the distribution of GRP94 development human embryonic organs.Method 9 human fetuses(weeks 10 31 of gestation)were studied by immunohistochemistry.Results The expression of GRP94 had been shown in the epithelial cells of colonic mucosa,renal tubule and hepatocytes,localized in the cytoplasma of the cells.GRP94 was observed at gestational week 10,increased markedly from 18 20 week and maintained at constantly high level.It was also detected in the gastric mucosa and cardiac muscle.No GRP94 expression was shown in lung skeletal muscle spinal cord and thymus.Conclusions GRP94 is constitutively expressed within specific tissues and suggest that GRP94 might play an important role in the embryogenesis.