银杏叶提取物对溶血卵磷脂胆碱致血管内皮细胞损伤的保护作用

应用传代培养的小牛主动脉内皮细胞(BAEC)为实验模型,观察银杏叶提取物(EGb)对溶血卵磷脂胆碱(LPC)损伤BAEC及其分泌功能的影响。结果发现培养BAEC与LPC(2.5μg·mL^-1)孵育24h后,出现细胞皱缩成团,大多细胞脱落、甚至细胞解体。细胞内漏出LDH增加,脂质过氧化物(MDA)含量增高,SOD活性降低,而PAI活性增高。LPC与不同浓度EGb共孵育24h,大部分细胞排列规则,形态基本正常,LDH明显减少,MDA含量下降,SOD活性增高及PAI活性降低。结果表明LPC可致培养血管内皮细胞损伤,而EGb可对抗LPC所造成的血管内皮细胞损伤,可能通过清除氧自由基、抗脂质过氧化作用和提高EC产生抗氧化能力。

PROTECTIVE EFFECTS OF EXTRACT OF GINKGO BILOBA (EGb) ON LYSOPHOSPHATIDYLCHOLINE INDUCED DAMAGES OF VASCULAR ENDOTHELIAL CELLS IN VITRO

Effects of EGb on cultured bovine aortic endothelial cells (BAEC) damaged by lysophosphatidylcholine (LPC) were investigated. When endothelial cells were incubated with LPC (2.5 micrograms.ml-1) for 24 h, the endothelial cells turned round pyknotic, exfoliated and broken, and the contents of malondialdehyde (MDA), lactate dehydrogenase (LDH) and plasminogen activator inhibitor (PAI) were markedly increased and the superoxide dismutase (SOD) activity was inhibited. Treatment with EGb at concentrations of 125 and 250 micrograms.ml-1, caused a reduction in MDA, LDH and PAI contents, while the SOD activity in the medium was increased and the morphologically damaged BAECs were alleviated. These results suggest that EGb afforded protection against BAECs damages induced by LPC and that the protective effect of EGb may be due to anti-lipid peroxidation via free-radical scavenging activity.