可降解聚对二氧环己酮网的制备、优化及体内降解研究

目的：制备聚对二氧环己酮（polydioxanone,PDO）网状人工补片,并通过体内降解实验研究,观察其降解过程及组织反应情况,为临床应用提供实验依据。方法：采用平纹机织方法将PDO纤维编织成网,并分别对其进行胶原及壳聚糖涂层处理后,分3组（单纯PDO网、胶原涂层PDO网和壳聚糖涂层PDO网）植入兔背部脊柱两侧肌肉内,于2、4、8、12、16、24周取材,动态观察材料与组织结合界面、降解吸收过程以及涂层对PDO网生物学特性的影响,并行组织学和透射电镜检查以观察组织反应。结果：PDO网植入体内炎性反应轻,纤维组织长入材料网孔间隙并与之结合紧密,8-12周后材料逐步降解,24周内完全降解吸收,材料周围未见变性、坏死或肉芽异常增生现象。胶原涂层促纤维组织生长包裹植入材料,组织随胶原逐步吸收而逐步长入材料网孔;壳聚糖涂层对植入材料周围纤维组织生长具有显著抑制作用。结论：聚对二氧杂环己酮网生物相容性佳,组织-材料界面友好,降解速度与组织再生相匹配,并可通过涂层处理改善其生物学性能,是一种具有临床应用前景的新型可降解人工补片材料。

Biodegradable polydioxanone mesh: preparation,optimization and in vivo degradation

Objective:To prepare a novel surgery mesh made of polydioxanone(PDO) and to study its in vivo degradation properties and the related tissue reaction,so as to provide reference for future clinical application.Methods: PDO mesh was plain-woven and was coated with collagen and chitosan,then they were implanted into the dorsal muscle of rabbits for 2,4,8,12,16,and 24 weeks.Rabbits implanted with pure PDO mesh served as control.The degradation process of implanted materials,tissue-material interface,and the biological influence of the coating on the mesh were observed;tissue reaction was observed and evaluated by histological means and TEM.Results: Implantation of pure PDO mesh resulted in mild inflammation and fibrous tissues infiltrated into the mesh;degradation occurred during the 8th to the 12th week and degraded completely within 24 weeks;there was no denaturalization,necrosis,and abnormal hyperplasia in the surrounding tissues.Collagen coating stimulated a fibroblastic proliferation and was reabsorbed within 4 weeks;and the naked mesh was infiltrated by fibrous tissues.The chitosan coating showed a strong inhibitory effect on the growth of the surrounding connective tissues.Conclusion: The biodegradable polydioxanone mesh has an excellent biocompatibility,a friendly tissue-material interface and a proper degradation rate matching to the tissue regeneration rate.The biological characteristics of the mesh can be modified by different coatings,making it a very promising degradable surgical mesh.