| 人参皂苷单体Rh_2促使人脑胶质瘤U251细胞凋亡的实验研究 |
| |
| 引用本文： | 邱建武,罗俊生,顾立学,关宁,张继全. 人参皂苷单体Rh_2促使人脑胶质瘤U251细胞凋亡的实验研究[J]. 辽宁中医杂志, 2011, 0(4): 773-776 |
| |
| 作者姓名： | 邱建武 罗俊生 顾立学 关宁 张继全 |
| |
| 作者单位： | 辽宁医学院附属第一医院;辽宁医学院; |
| |
| 基金项目： | 辽宁省教育厅基金资助项目(2009A448); 锦州市科学技术局资助项目(05B1E01) |
| |
| 摘 要： | 目的:观察人参皂苷单体Rh2(GS-Rh2)诱导人脑胶质瘤U251细胞的凋亡作用,进而探讨其对端粒酶活性的影响和人端粒酶逆转录酶(hTERT)表达的机制。方法:人脑胶质瘤U251细胞经GS-Rh2处理后,采用MTT法检测细胞抑制率,免疫荧光技术观察凋亡小体,流式细胞仪经Annexin V-FITC/PI双染法观察细胞凋亡,TRAP-ELISA法检测端粒酶活性改变,通过RT-PCR技术检测hTERT基因的表达。结果:GS-Rh2对U251细胞具有抑制作用,呈明显的剂量依赖关系,免疫荧光技术观察到凋亡小体的出现,流式细胞仪检测GS-Rh2引起U251细胞凋亡并与药物浓度存在依赖关系,TRAP-ELISA法发现GS-Rh2可以降低端粒酶活性且呈剂量和时间依赖关系,RT-PCR证实GS-Rh2可降低hTERT基因的表达。结论:人参皂苷单体Rh2能够诱导人脑胶质瘤U25l细胞发生凋亡,并抑制和降低细胞端粒酶活性,经过RT-PCR证实GS-Rh2作用于胶质瘤的分子生物学机制可能与抑制胶质瘤细胞hTERT基因有关,进而诱导细胞发生凋亡。
|
| 关 键 词： | 人参皂苷单体Rh2 胶质瘤 细胞凋亡 hTERT mRNA |
The Studies on the Apoptosis of Human Glioma U251 Cells Induced By GS-Rh_2 |
| |
| QIU Jian-wu,LUO Jun-sheng,GU Li-xue,GUAN Ning,ZHANG Ji-quan. The Studies on the Apoptosis of Human Glioma U251 Cells Induced By GS-Rh_2[J]. Liaoning Journal of Traditional Chinese Medicine, 2011, 0(4): 773-776 |
| |
| Authors: | QIU Jian-wu LUO Jun-sheng GU Li-xue GUAN Ning ZHANG Ji-quan |
| |
| Affiliation: | QIU Jian-wu1,LUO Jun-sheng1,GU Li-xue1,GUAN Ning1,ZHANG Ji-quan2(1.The First Affiliated Hospital of Liaoning Medical University,Jinzhou 121001,Liaoning,China,2.Liaoning Medical University,China) |
| |
| Abstract: | Objective:To observe the mechanism of cellular apoptosis of human glioma U251 cells and investigate the effect of telomerase activity and the expression of human telomerase reverse transcripase(hTERT) by ginsenoside-Rh2(GS-Rh2).Methods:Human glioma U251 cells were treated with GS-Rh2,the inhibitory effect was then examined using MTT.To observe the apoptotis body by immunofluorescence and light microscopy.Staining with Annexin V-FITC/PI is at present a useful method to detect the change of apoptotic cells by... |
| |
| Keywords: | GS-Rh2 Glioma Apoptosis hTERT mRNA |
| 本文献已被 CNKI 维普 等数据库收录！ |
|
