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| 新城疫病毒NP蛋白的真核表达载体的构建 | |
| 引用本文： | 于健,王久莉,孙琦,陈奖励,宋秀龙,李树祥.新城疫病毒NP蛋白的真核表达载体的构建[J].哈尔滨医科大学学报,2000,34(5):323-325. |
| 作者姓名： | 于健 王久莉 孙琦 陈奖励 宋秀龙 李树祥 |
| 作者单位： | 1. 哈尔滨医科大学附属第二医院,耳鼻喉科,黑龙江,哈尔滨,150086 2. 中国农科院哈尔滨兽医研究所,黑龙江,哈尔滨,150020 3. 哈尔滨医科大学,病理学教研室,黑龙江,哈尔滨,150086 |
| 基金项目： | 国家自然科学基金，96， |
| 摘 要： | 目的 研究新城疫病毒核衣壳蛋白基因的生物学作用及其抗人喉鳞癌的作用。方法 利用含有新城疫病毒Ｖ４株核衣壳蛋白基因ｃＤＮＡ的原核质粒ｐＵＶＮＰ４及真核质料ｐｃＮＤＡ３，采用基因重组技术构建了含有ＣＭＶ含动子、ＢＧＨｐｏｌｙＡ信号序列的表达新城疫病毒核衣壳蛋白的真核表达质粒。结果 酶切分析表明重组质粒中含有新城疫病毒Ｖ４株核衣壳蛋白基因。结论 重组质粒为表达新城疫病毒核衣壳蛋白基因的真核质粒。 |
| 关 键 词： | 新城疫病毒 真核表达载体 NP蛋白 喉鳞癌 |
Construction of eukaryotic vector of the nucleocapsid protein gene of newcastle disease virus |
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| YU Jian,WANG Jiu li,SUN Qi,et al.Construction of eukaryotic vector of the nucleocapsid protein gene of newcastle disease virus[J].Journal of Harbin Medical University,2000,34(5):323-325. | |
| Authors: | YU Jian WANG Jiu li SUN Qi |
| Abstract: | Objective To study the biology function and effect of Newcastle disease virus(NDV) in human laryngeal squamous carcinoma.Methods The cloning plasmid (pUVNP 4) which contain the nucleocapsid protein gene of NDV strain V 4 and the eukaryotic plasmid (pcDNA 3)which contain CMV promoter and signal sequence of BGH poly A were used for construction of the new eukaryotic plasmid,it expressed the nucleocapsid protain in the eukaryotic cell. The present study has been carried out using the gene recombination technique. Results Restriction endonuclease analysis showed that is a specific cloning plasmid of contained the nucleocapsid protein gene of NDV strain V 4. Conclusion The recombinant is the eukaryotic plasmid included the nucleocapsid protein gene of NDV strain V 4. |
| Keywords: | Newcastle disease virus nucleocapsid protein gene eukaryotic vector construction |
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