| NK、T混合淋巴细胞与顺铂联合对乳腺癌MDA-MB-231细胞株体外杀伤研究 |
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| 引用本文： | 游俊浩,杨俊兰,焦顺昌,林星石,吴亮亮,王刚.NK、T混合淋巴细胞与顺铂联合对乳腺癌MDA-MB-231细胞株体外杀伤研究[J].军医进修学院学报,2009,30(3):378-379. |
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| 作者姓名： | 游俊浩 杨俊兰 焦顺昌 林星石 吴亮亮 王刚 |
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| 作者单位： | 解放军总医院肿瘤科,北京,100853 |
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| 摘 要： | 目的：探讨利用荧光染料CFSE标记法及MTT法,观察人NK、T混合淋巴细胞（NKTm）体外杀伤MDA-MB-231肿瘤株的效果。方法：应用CFSE/PI染色法及MTT法标记人乳腺癌MDA-MB-231肿瘤株,观察NKT细胞联合顺铂在各种时序下对乳腺癌MDA-MB-231肿瘤株的杀伤效果。结果：CFSE染色条件下,人NKTm细胞体外对MDA-MB-231细胞效靶比E/T=25：1时较E/T=12.5：1时杀伤率明显升高;E/T=12.5：1时杀伤率略低于顺铂;先加入NKTm各组随与顺铂间隔时间的延长而杀伤率升高（以上P均〈0.05）。结论：NKTm细胞对MDA-MB-231肿瘤细胞的直接杀伤作用强,同顺铂联用时要提高对肿瘤细胞的杀伤率应先给予NKTm细胞后应用顺铂。
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| 关 键 词： | 杀伤细胞 天然 乳腺肿瘤 细胞 CFSE 免疫治疗 顺铂 |
Combined cytotoxic effects of NK and T lymphocytes and cisplatin on human breast cancer cell line MDA-MB-231 in vitro |
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| YOU Jun-hao,YANG Jun-lan,JIAO Shun-chang,LIN Xing-shi,WU Liang-liang,WANG Gang.Combined cytotoxic effects of NK and T lymphocytes and cisplatin on human breast cancer cell line MDA-MB-231 in vitro[J].Academic Journal of Pla Postgraduate Medical School,2009,30(3):378-379. |
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| Authors: | YOU Jun-hao YANG Jun-lan JIAO Shun-chang LIN Xing-shi WU Liang-liang WANG Gang |
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| Institution: | Department of Medical Oncology;Chinese PLA General Hospital;Beijing 100853;China |
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| Abstract: | Objective:To investigate the combined effects of NK and T lymphocytes(NKTm cells) and cisplatin(DDP) on CFSE labeled human breast cancer cell line MDA-MB-231.Methods:CFSE/PI labeled cell line MDA-MB-231 was treated with NKTm cells or with NKTm cells and cisplatin.Cytotoxic activity was determined by MTT colorimetric assay and flow cytometry with specific antibodies.Results:The NKTm cells showed their cytotoxic effects on CFSE labeled cell line MDA-MB-231 in a concentration-dependent manner(P<0.05),The inhib... |
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| Keywords: | killer cells natural breast neoplasms cells CFSE immunotherapy cisplatin |
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