吲哚胺2,3-双加氧酶在尖锐湿疣皮损中表达的研究

目的 分析尖锐湿疣组织中吲哚胺2,3-双加氧酶(IDO)水平,研究其局部代谢色氨酸的能力.方法 免疫组化法观察尖锐湿疣患者皮肤IDO蛋白表达情况,计数IDO阳性细胞的比例.免疫荧光观察IDO(+)细胞与树突细胞的关系.分离对照皮肤角质形成细胞和疣体上皮细胞,色氨酸体外孵育后检测上清液中色氨酸代谢产物犬尿氨酸的浓度以反映细胞代谢色氨酸的能力.结果IDO(+)细胞在正常皮肤中非常少,但在疣体表皮中却大量聚集.疣体内IDO(+)细胞/总体细胞48.3%±15.4%显著高于正常皮肤5.2%±2.4%,差异有统计学意义(P<0.05).IDO(+)细胞的荧光信号和皮肤朗格汉斯细胞并不重合,提示来源于疣体表皮细胞.从疣体组织分离的上皮细胞在体外代谢色氨酸的能力强于健康对照皮肤中分离的表皮细胞.结论 尖锐湿疣疣体中存在大量的IDO(+)细胞,这些细胞可能参与尖锐湿疣的发病.

Expression of indoleamine 2, 3-dioxygenase in condyloma acuminatum lesions

Objective To measure the expression of indoleamine 2, 3-dioxygenase(IDO)in condy-loma acuminatum (CA) lesions, and to evaluate its ability to locally metabolize tryptophan. Methods Immunohistochemical study was performed to observe the protein expression of IDO in skin lesions of patients with CA, and count the number of IDO-positive cells. Immunofluorescence assay was conducted to estimate the relationship between IDO-positive cells and dendritic cells. Epidermal cells and keratinocytes were isolated from warts of 30 patients with CA and prepuces of 11 healthy controls respectively, and both in vitro incubated with tryptophan solution for 4 hours. Then, high-performance liquid chromatography (HPLC)was performed to detect the level of tryptophan metabolite, kynurenine, in the culture supernatant of the above cells, which could reflect the ability of epidermal cells to metabolize tryptophan. Results Rare IDO-positive cells were found in the normal skin, but a lot of IDO-positive cells gathered in the epidermis of the wart tissues. The IDO-positive cell/total cell ratio was significantly higher in the wart tissues than in the normal skin(48.3%± 15.4%vs. 5.2%± 2.4%, P<0.05). The fluorescence signals of IDO-positive cells and CD1a-positive Langerhans cells were not overlapped with each other, suggesting that IDO-positive cells were derived from epidermal cells of the wart tissues. Compared with the keratinocytes from the healthy skin, the epidermal cells from warts had a stronger ability to metabolize tryptophan in vitro. Conclusion A large number of IDO-positive cells exist in CA warts, and may be involved in occurrence of CA.